Cloned (Comment) | Organism |
---|---|
recombinant expression in Escherichia coli | Acidothermus cellulolyticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.35 | - |
oat spelt xylan | pH 6.0, 90°C | Acidothermus cellulolyticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidothermus cellulolyticus | - |
- |
- |
Acidothermus cellulolyticus 11B | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli by heat treatment at 65°C for 15 min and hydroxyapatite chromatography | Acidothermus cellulolyticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
birch wood xylan + H2O | best substrate, thin-layer chromatography determination of reaction products, the major products from oat spelt and birchwood xylan have retention factor values between those of xylose and xylopentaose | Acidothermus cellulolyticus | ? | - |
? | |
birch wood xylan + H2O | best substrate, thin-layer chromatography determination of reaction products, the major products from oat spelt and birchwood xylan have retention factor values between those of xylose and xylopentaose | Acidothermus cellulolyticus 11B | ? | - |
? | |
additional information | Xyn10A is an endoxylanase. No activity with cellulose, carboxymethyl cellulose, and xanthan gum | Acidothermus cellulolyticus | ? | - |
? | |
additional information | Xyn10A is an endoxylanase. No activity with cellulose, carboxymethyl cellulose, and xanthan gum | Acidothermus cellulolyticus 11B | ? | - |
? | |
oat spelt xylan + H2O | thin-layer chromatography determination of reaction products, the major products from oat spelt and birchwood xylan have retention factor values between those of xylose and xylopentaose | Acidothermus cellulolyticus | ? | - |
? | |
oat spelt xylan + H2O | thin-layer chromatography determination of reaction products, the major products from oat spelt and birchwood xylan have retention factor values between those of xylose and xylopentaose | Acidothermus cellulolyticus 11B | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme is a family 10 xylanase | Acidothermus cellulolyticus |
Xyn10A | - |
Acidothermus cellulolyticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
- |
Acidothermus cellulolyticus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 100 | activity range, pH-dependent: at 100°C activity occurs at pH 4.0-6.0, at 60°C activity occurs at pH 5.0-8.0, overview | Acidothermus cellulolyticus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
half-life is 1.5 h in presence of xylan | Acidothermus cellulolyticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Acidothermus cellulolyticus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | activity range, at pH 8.0 activity occurs only up to 70°C, at pH 7.0 up to 90°C, at pH 4.0 activity occurs at 90-100°C, overview | Acidothermus cellulolyticus |