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Literature summary for 3.2.1.8 extracted from

  • Dobrev, G.; Zhekova, B.; Delcheva, G.; Koleva, L.; Tziporkov, N.; Pishtiyski, I.
    Purification and characterization of endoxylanase Xln-1 from Aspergillus niger B03 (2009), World J. Microbiol. Biotechnol., 25, 2095-2102.
    View publication on PubMed
Search for more literature for 3.2.1.8

Inhibitors

Inhibitors Comment Organism Structure
Ag+ weak inhibition Aspergillus niger
Cd(CH3COO)2
-
 Aspergillus niger
Cu2+ complete inhibition at 5 mM Aspergillus niger
Fe2+
-
 Aspergillus niger
Fe3+
-
 Aspergillus niger
K+ weak inhibition Aspergillus niger
Li+ weak inhibition Aspergillus niger
Mg2+ weak inhibition Aspergillus niger
Na+ weak inhibition Aspergillus niger
Pb(CH3COO)2
-
 Aspergillus niger
Pb(NO3)2
-
 Aspergillus niger
Zn2+
-
 Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km for oat spelt xylan is 8.19 mg/ml, kinetics Aspergillus niger

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Aspergillus niger
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates weakly Aspergillus niger
Mn2+ activates Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 1 * 33000, SDS-PAGE Aspergillus niger
34000
-
 gel filtration Aspergillus niger

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-1,4-xylan + H2O Aspergillus niger
-
 ?
-
 ?
beta-1,4-xylan + H2O Aspergillus niger B03
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-
Aspergillus niger B03
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the degree of glycosylation is 74.1% Aspergillus niger

Purification (Commentary)

Purification (Comment) Organism
native enzyme 4fold by consecutive ultrafiltration and anion exchange chromatography Aspergillus niger

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
475.1
-
 purified native enzyme, beechwood xylan Aspergillus niger
497.2
-
 purified native enzyme, birchwood xylan Aspergillus niger
529.9
-
 purified native enzyme, oat spelt xylan Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-1,4-xylan + H2O
-
 Aspergillus niger ?
-
 ?
beta-1,4-xylan + H2O from oat spelt, birchwood, or beechwood Aspergillus niger ?
-
 ?
beta-1,4-xylan + H2O
-
 Aspergillus niger B03 ?
-
 ?
beta-1,4-xylan + H2O from oat spelt, birchwood, or beechwood Aspergillus niger B03 ?
-
 ?
additional information endo mechanism of Xln-1 and substrate specificity, overview, no activity with carboxymethyl cellulose, starch, pectin, and 4-nitrophenyl-beta-D-xylopyranoside, and 4-nitrophenyl-beta-D-glucopyranoside Aspergillus niger ?
-
 ?
additional information endo mechanism of Xln-1 and substrate specificity, overview, no activity with carboxymethyl cellulose, starch, pectin, and 4-nitrophenyl-beta-D-xylopyranoside, and 4-nitrophenyl-beta-D-glucopyranoside Aspergillus niger B03 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 33000, SDS-PAGE Aspergillus niger

Synonyms

Synonyms Comment Organism
endoxylanase
-
 Aspergillus niger
Xln-1
-
 Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Aspergillus niger

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70 activity range, profile, overview Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 purified native enzyme, pH 7.0, 120 min, 80% activity remaining Aspergillus niger
50
-
 purified native enzyme, pH 5.0-7.0, 86% residual activity after 45 min, the crude enzyme retains only 6% of the initial activity Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Aspergillus niger

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 7 purified native enzyme, 50°C, 86% residual activity after 45 min, the crude enzyme retains only 6% of the initial activity Aspergillus niger
7
-
 purified native enzyme, 40°C, 120 min, 80% activity remaining Aspergillus niger