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Literature summary for 3.2.1.8 extracted from
- Thermostable carbohydrate binding module increases the thermostability and substrate-binding capacity of Trichoderma reesei xylanase 2 (2009), New Biotechnol., 26, 53-59.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of gene xyn2 and hybrid gene in Pichia pastoris under control of the inducible alcohol oxidase 1, AOX1, promoter using the secretion signal sequence from Saccharomyces cerevisiae, subcloning in Escherichis coli strain DH5alpha | Trichoderma reesei |
| expression of the hybrid gene in Pichia pastoris under control of the inducible alcohol oxidase 1, AOX1, promoter using the secretion signal sequence from Saccharomyces cerevisiae, subcloning in Escherichia coli strain DH5alpha | Thermotoga maritima |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | improving the thermostability of Trichoderma reesei xylanase 2 by introducing the thermostabilizing domain A2 from Thermotoga maritima XynA into the N-terminal region of the Xyn2 protein | Trichoderma reesei |
| additional information | improving the thermostability of Trichoderma reesei xylanase 2 by introducing the thermostabilizing domain A2 from Thermotoga maritima XynA into the N-terminal region of the Xyn2 protein | Thermotoga maritima |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26000 | - |
x * 20000-21000, recombinant wild-type enzyme, SDS-PAGE, x * 26000, mutant hybrid enzyme, SDS-PAGE | Trichoderma reesei |
| 26000 | - |
x * 26000, mutant hybrid enzyme, SDS-PAGE | Thermotoga maritima |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-1,4-xylan + H2O | Trichoderma reesei | - |
? | - |
? |  |
| beta-1,4-xylan + H2O | Thermotoga maritima | - |
? | - |
? | |
| beta-1,4-xylan + H2O | Trichoderma reesei RUT C-30 | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermotoga maritima | - |
gene XynA | - |
| Trichoderma reesei | - |
gene xyn2 | - |
| Trichoderma reesei RUT C-30 | - |
gene xyn2 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-1,4-xylan + H2O | - |
Trichoderma reesei | ? | - |
? | |
| beta-1,4-xylan + H2O | - |
Thermotoga maritima | ? | - |
? | |
| beta-1,4-xylan + H2O | - |
Trichoderma reesei RUT C-30 | ? | - |
? | |
| beta-1,4-xylan + H2O | from birchwood | Trichoderma reesei | xylobiose + xylotriose + ? | the main degradation products | ? | |
| beta-1,4-xylan + H2O | from birchwood | Thermotoga maritima | xylobiose + xylotriose + ? | the main degradation products | ? | |
| beta-1,4-xylan + H2O | from birchwood | Trichoderma reesei RUT C-30 | xylobiose + xylotriose + ? | the main degradation products | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 20000-21000, recombinant wild-type enzyme, SDS-PAGE, x * 26000, mutant hybrid enzyme, SDS-PAGE | Trichoderma reesei |
| ? | x * 26000, mutant hybrid enzyme, SDS-PAGE | Thermotoga maritima |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| xylanase 2 | - |
Trichoderma reesei |
| xylanase A | - |
Thermotoga maritima |
| Xyn2 | - |
Trichoderma reesei |
| XynA | - |
Thermotoga maritima |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
recombinant wild-type enzyme | Trichoderma reesei |
| 65 | - |
recombinant hybrid mutant | Trichoderma reesei |
| 65 | - |
recombinant hybrid mutant | Thermotoga maritima |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 90 | activity range of mutant hybrid enzyme, profile, overview | Thermotoga maritima |
| 30 | 90 | activity range of wild-type and mutant enzymes, profile, overview, 30% of maximal activity at 75°C | Trichoderma reesei |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
recombinant mutant hybrid enzyme, 30 min, over 85% activity remaining | Trichoderma reesei |
| 60 | - |
recombinant mutant hybrid enzyme, 30 min, over 85% activity remaining | Thermotoga maritima |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
assay at | Trichoderma reesei |
| 5 | - |
assay at | Thermotoga maritima |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2 | 9 | profile, overview | Trichoderma reesei |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the N-terminal domain A2 is responsible for both the thermostability and substrate-binding capacity of XynA | Thermotoga maritima |
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