Cloned (Comment) | Organism |
---|---|
gene xylA, DNA and amino acid sequence determination and analysis, expression in Escherichia coli | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | slight inhibition | Bacillus subtilis | |
Cu2+ | - |
Bacillus subtilis | |
EDTA | slight inhibition | Bacillus subtilis | |
Fe2+ | - |
Bacillus subtilis | |
Mn2+ | - |
Bacillus subtilis | |
SDS | slight inhibition | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
x * 23253, sequence calculation, x * 23000, SDS-PAGE | Bacillus subtilis |
23253 | - |
x * 23253, sequence calculation, x * 23000, SDS-PAGE | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-1,4-xylan + H2O | Bacillus subtilis | - |
xylobiose + xylotriose + ? | the predominant products resulting from xylan and xylooligosaccharide hydrolysis are xylobiose and xylotriose. The enzyme can hydrolyze xylooligosaccharides larger than xylotriose | ? | |
beta-1,4-xylan + H2O | Bacillus subtilis AMX-4 | - |
xylobiose + xylotriose + ? | the predominant products resulting from xylan and xylooligosaccharide hydrolysis are xylobiose and xylotriose. The enzyme can hydrolyze xylooligosaccharides larger than xylotriose | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
isolated from soil, gene xylA | - |
Bacillus subtilis AMX-4 | - |
isolated from soil, gene xylA | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arabinoxylan + H2O | - |
Bacillus subtilis | ? | - |
? | |
arabinoxylan + H2O | - |
Bacillus subtilis AMX-4 | ? | - |
? | |
beta-1,4-xylan + H2O | - |
Bacillus subtilis | xylobiose + xylotriose + ? | the predominant products resulting from xylan and xylooligosaccharide hydrolysis are xylobiose and xylotriose. The enzyme can hydrolyze xylooligosaccharides larger than xylotriose | ? | |
beta-1,4-xylan + H2O | from oat spelt or birchwood | Bacillus subtilis | xylobiose + xylotriose + ? | the predominant products resulting from xylan and xylooligosaccharide hydrolysis are xylobiose and xylotriose. The enzyme can hydrolyze xylooligosaccharides larger than xylotriose | ? | |
beta-1,4-xylan + H2O | - |
Bacillus subtilis AMX-4 | xylobiose + xylotriose + ? | the predominant products resulting from xylan and xylooligosaccharide hydrolysis are xylobiose and xylotriose. The enzyme can hydrolyze xylooligosaccharides larger than xylotriose | ? | |
beta-1,4-xylan + H2O | from oat spelt or birchwood | Bacillus subtilis AMX-4 | xylobiose + xylotriose + ? | the predominant products resulting from xylan and xylooligosaccharide hydrolysis are xylobiose and xylotriose. The enzyme can hydrolyze xylooligosaccharides larger than xylotriose | ? | |
additional information | AMX-4 xylanase is not active toward other polysaccharides including carboxymethylcellulose, locust bean gum, lichenan, laminarin, and Avicel or toward synthetic substrate derivatives such as 4-nitrophenyl-beta-xylopyranoside, 4-nitrophenyl-beta-mannoside, and 4-nitrophenyl-beta-cellobioside, it can hydrolyze only beta-1,4-xylosidic linkages and has no beta-xylosidase activity | Bacillus subtilis | ? | - |
? | |
additional information | AMX-4 xylanase is not active toward other polysaccharides including carboxymethylcellulose, locust bean gum, lichenan, laminarin, and Avicel or toward synthetic substrate derivatives such as 4-nitrophenyl-beta-xylopyranoside, 4-nitrophenyl-beta-mannoside, and 4-nitrophenyl-beta-cellobioside, it can hydrolyze only beta-1,4-xylosidic linkages and has no beta-xylosidase activity | Bacillus subtilis AMX-4 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 23253, sequence calculation, x * 23000, SDS-PAGE | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
AMX-4 xylanase | - |
Bacillus subtilis |
More | the enzyme belongs to glycosyl hydrolase family 11, GH11 | Bacillus subtilis |
xylanase | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 55 | - |
Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | - |
Bacillus subtilis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bacillus subtilis | - |
- |
9.27 |