Cloned (Comment) | Organism |
---|---|
gene xynC, subcloning in Escherichia coli strain DH5alpha, expression of wild-type and mutant enzymes in Escherichia coli | Acetivibrio thermocellus |
gene xynZ, subcloning in Escherichia coli strain DH5alpha, expression of wild-type and mutant enzymes in Escherichia coli | Acetivibrio thermocellus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of two constructs of XynC, one with its cellulose binding domain and the catalytic domain, pXynC-BC, and the other with only the catalytic domain, pXynC-C. The specific activities of XynC with and without the non-catalytic domains are similar | Acetivibrio thermocellus |
additional information | generation of two constructs of XynZ: pXynZ-BDC, which includes the dockerin domain, and pXynZ-C, which does not. For XynZ, the specific activity of the enzyme without the non-catalytic domains is about 5fold greater than that of the intact enzyme. The overall increase in activity is 9fold higher for XynZ-C versus XynZ-BDC | Acetivibrio thermocellus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km values for mutants XynC-BC and XynC-C determined on soluble xylan are 3.1 mg/ml and 3.6 mg/ml, respectively | Acetivibrio thermocellus | |
additional information | - |
additional information | Km values for mutants XynZ-BDC and XynZ-C, determined on soluble xylan, are 33.3 mg/ml and 15.4 mg/ml, respectively | Acetivibrio thermocellus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-1,4-xylan + H2O | Acetivibrio thermocellus | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetivibrio thermocellus | O32374 | xynC; strain ATCC 27405D, gene xynC | - |
Acetivibrio thermocellus | P10478 | xynZ; strain ATCC 27405D, gene xynZ | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activities of wild-type XynC, and its domain deletion mutants, overview | Acetivibrio thermocellus |
additional information | - |
activities of wild-type XynZ, and its domain deletion mutants, overview | Acetivibrio thermocellus |
3 | 8 | purified mutant XynC-BC | Acetivibrio thermocellus |
39 | - |
purified mutant XynC-C | Acetivibrio thermocellus |
79 | - |
purified mutant XynZ-BDC | Acetivibrio thermocellus |
390 | - |
purified mutant XynZ-C | Acetivibrio thermocellus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-1,4-xylan + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
beta-1,4-xylan + H2O | from birchwood | Acetivibrio thermocellus | ? | - |
? | |
beta-1,4-xylan + H2O | insoluble xylan from birchwood, and soluble xylan | Acetivibrio thermocellus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
xylanase | - |
Acetivibrio thermocellus |
XynC | - |
Acetivibrio thermocellus |
XynZ | - |
Acetivibrio thermocellus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Acetivibrio thermocellus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
2 h, 87% activity of mutant Xyn-BDC is remaining, 42% of mutant Xyn-BDC | Acetivibrio thermocellus |
70 | - |
2 h, mutant Xyn-C loses all activity, while mutant XynC-BC retains 70% activity | Acetivibrio thermocellus |