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Literature summary for 3.2.1.8 extracted from

  • Adsul, M.G.; Bastawde, K.B.; Gokhale, D.V.
    Biochemical characterization of two xylanases from yeast Pseudozyma hubeiensis producing only xylooligosaccharides (2009), Biores. Technol., 100, 6488-6495.
    View publication on PubMed
Search for more literature for 3.2.1.8

Inhibitors

Inhibitors Comment Organism Structure
1-ethyl-3-(3-dimethylaminopropyl) carbodiimide at 100 mM 20% inhibition of PhX20, 30% inhibition of PhX33 Pseudozyma hubeiensis
2,4,6-Trinitrobenzenesulfonic acid at 1 mM 90% inhibition of PhX20, 30% inhibition of PhX33 Pseudozyma hubeiensis
citraconic anhydride at 50 mM no inhibition of PhX20, 10% inhibition of PhX33 Pseudozyma hubeiensis
diethyldicarbonate at 5 mM 95% inhibition of PhX20, 10% inhibition of PhX33 Pseudozyma hubeiensis
additional information no or poor inhibition of PhX33 and PhX20 by N-ethylmaleimide, iodoacetate, 2,3-butanedione, and N-acetylimidazole Pseudozyma hubeiensis
N-bromosuccinimide complete inhibition of PhX33 and PhX20 at 1 mM Pseudozyma hubeiensis
p-chloromercuribenzoate at 50 mM 50% inhibition of PhX20, 40% inhibition of PhX33 Pseudozyma hubeiensis
Phenylglyoxal at 10 mM 25% inhibition of PhX20, no inhibition of PhX33 Pseudozyma hubeiensis
Phenylmethylsulfonylfluoride at 10 mM 15% inhibition of PhX20, 10% inhibition of PhX33 Pseudozyma hubeiensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km for PhX33 and PhX20 is 7.6 mg/ml and 11.1 mg/ml, respectively using oat-spelt soluble xylan as substrate Pseudozyma hubeiensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20100
-
 x * 33500, about, PhX33, mass spectrometry, x * 20100, about, PhX20, mass spectrometry Pseudozyma hubeiensis
33500
-
 x * 33500, about, PhX33, mass spectrometry, x * 20100, about, PhX20, mass spectrometry Pseudozyma hubeiensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-1,4-xylan + H2O Pseudozyma hubeiensis
-
 xylooligosaccharides
-
 ?
beta-1,4-xylan + H2O Pseudozyma hubeiensis NCIM 3574
-
 xylooligosaccharides
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudozyma hubeiensis
-
 isolated from decaying sandal wood
-
Pseudozyma hubeiensis NCIM 3574
-
 isolated from decaying sandal wood
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein PHX20 and PhX33 Pseudozyma hubeiensis

Purification (Commentary)

Purification (Comment) Organism
native enzymes by anion exchange chromatography and gel filtration, PhX33 3.29fold, PhX20 5.73fold Pseudozyma hubeiensis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
407
-
 purified PhX33 Pseudozyma hubeiensis
710
-
 purified PhX20 Pseudozyma hubeiensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-1,4-xylan + H2O
-
 Pseudozyma hubeiensis xylooligosaccharides
-
 ?
beta-1,4-xylan + H2O
-
 Pseudozyma hubeiensis NCIM 3574 xylooligosaccharides
-
 ?
beta-1,4-xylan + H2O PhX33 possesses three tryptophan and one carboxyl residues at the active site. The active site of PhX20 comprises one residue each of tryptophan, carboxyl and histidine Pseudozyma hubeiensis xylotriose + xylotetraose + xylopentaose + xylopentaose + xylohexaose + xyloheptaose both the xylanases PhX33 and PhX20 produce only xylooligosaccharides with degree of polymerization 3-7 without formation of xylose and xylobiose. PhX33 hydrolyzes xylan to xylotriose, xylotetraose and xylopentaose and PhX20 gives xylotriose, xylotetraose, xylopentaose, xylohexaose, and xyloheptaose ?
beta-1,4-xylan + H2O PhX33 possesses three tryptophan and one carboxyl residues at the active site. The active site of PhX20 comprises one residue each of tryptophan, carboxyl and histidine Pseudozyma hubeiensis NCIM 3574 xylotriose + xylotetraose + xylopentaose + xylopentaose + xylohexaose + xyloheptaose both the xylanases PhX33 and PhX20 produce only xylooligosaccharides with degree of polymerization 3-7 without formation of xylose and xylobiose. PhX33 hydrolyzes xylan to xylotriose, xylotetraose and xylopentaose and PhX20 gives xylotriose, xylotetraose, xylopentaose, xylohexaose, and xyloheptaose ?

Subunits

Subunits Comment Organism
? x * 33500, about, PhX33, mass spectrometry, x * 20100, about, PhX20, mass spectrometry Pseudozyma hubeiensis
More PhX33 contains predominant alpha-and, while PhX22 contains predominantly beta-sheets Pseudozyma hubeiensis

Synonyms

Synonyms Comment Organism
PhX20
-
 Pseudozyma hubeiensis
PhX33
-
 Pseudozyma hubeiensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 assay at Pseudozyma hubeiensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
55 60 PhX20 Pseudozyma hubeiensis
60 65 PhX33 Pseudozyma hubeiensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 purified PhX20, half-life is 10 min Pseudozyma hubeiensis
70
-
 purified PhX33, half-life is 15 min Pseudozyma hubeiensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1383
-
 beta-1,4-xylan pH 7.0, 60°C, PhX33, oat spelt xylan Pseudozyma hubeiensis
3333
-
 beta-1,4-xylan pH 7.0, 60°C, PhX20, oat spelt xylan Pseudozyma hubeiensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2
-
 PhX20, isoelectric focusing Pseudozyma hubeiensis
4.7 6 PhX33, isoelectric focusing Pseudozyma hubeiensis

pH Stability

pH Stability pH Stability Maximum Comment Organism
2 9 PhX20, 90% activity remains after 48 h Pseudozyma hubeiensis
3 9 PhX33, 90% activity remains after 48 h Pseudozyma hubeiensis