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Literature summary for 3.2.1.8 extracted from

  • Liu, W.; Shi, P.; Chen, Q.; Yang, P.; Wang, G.; Wang, Y.; Luo, H.; Yao, B.
    Gene cloning, overexpression, and characterization of a xylanase from Penicillium sp. CGMCC 1669 (2010), Appl. Biochem. Biotechnol., 162, 1-12.
    View publication on PubMed
Search for more literature for 3.2.1.8

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol activates by 35% at 10 mM Penicillium sp. CGMCC 1669

Cloned(Commentary)

Cloned (Comment) Organism
gene xyn11F63, DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli strain DH5alpha, overexpression in Pichia pastoris, the recombinant enzyme is secreted to the culture medium Penicillium sp. CGMCC 1669

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 90% inhibition at 5 mM Penicillium sp. CGMCC 1669
Co2+ 24% inhibition at 10 mM Penicillium sp. CGMCC 1669
Cr3+ 18% inhibition at 10 mM Penicillium sp. CGMCC 1669
Cu2+ 40% inhibition at 5-10 mM Penicillium sp. CGMCC 1669
Fe3+ 40% inhibition at 10 mM Penicillium sp. CGMCC 1669
Hg2+ complete inhibition at 5 mM Penicillium sp. CGMCC 1669
Mn2+ 10% inhibition at 1 mM Penicillium sp. CGMCC 1669
additional information no or poor inhibition by Na+, Li+, K+, Mg2+, and Ca2+ Penicillium sp. CGMCC 1669
Ni2+ 10% inhibition at 5-10 mM Penicillium sp. CGMCC 1669
Pb2+ 42% inhibition at 10 mM Penicillium sp. CGMCC 1669
SDS 54% inhibition at 1 mM, 92% at 10 mM Penicillium sp. CGMCC 1669

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics Penicillium sp. CGMCC 1669

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21500
-
 x * 21500, about, sequence calculation Penicillium sp. CGMCC 1669

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-1,4-xylan + H2O Penicillium sp. CGMCC 1669
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Penicillium sp. CGMCC 1669 D1GFE6 gene xyn11F63; gene xyn11F63
-

Purification (Commentary)

Purification (Comment) Organism
recombinantz enzyme 2.33fold from Pichia pastoris to homogeneity from culture medium Penicillium sp. CGMCC 1669

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7988
-
 purified recombinant enzyme Penicillium sp. CGMCC 1669

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-1,4-xylan + H2O
-
 Penicillium sp. CGMCC 1669 ?
-
 ?
beta-1,4-xylan + H2O from birchwood or oat spelt Penicillium sp. CGMCC 1669 D-xylose + 1,4-beta-D-xylobiose + 1,4-beta-D-xylotriose + 1,4-beta-D-xylotetraose from oat spelt xylan results 7.46% xylose, 28.36% xylobiose, 25.37% xylotriose, and 38.81% xylotetraose ?
additional information no activity towards carboxymethylcellulose-sodium, lichenan, or barley beta-glucan Penicillium sp. CGMCC 1669 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 21500, about, sequence calculation Penicillium sp. CGMCC 1669

Synonyms

Synonyms Comment Organism
XYN11F63
-
 Penicillium sp. CGMCC 1669

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 recombinant enzyme Penicillium sp. CGMCC 1669

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5416
-
 beta-1,4-xylan recombinant enzyme, pH 4.5, 37°C Penicillium sp. CGMCC 1669

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
 recombinant enzyme Penicillium sp. CGMCC 1669

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 9 purified recombinant enzyme, stable Penicillium sp. CGMCC 1669