Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | activates 1.6fold at 10 mM | Streptomyces sp. TN119 |
Cloned (Comment) | Organism |
---|---|
gene xynA119, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of the His-tagged enzyme in Escherichia coli strain BL21 (DE3), the recombinant xylanase is secreted to the culture supernatant | Streptomyces sp. TN119 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 19% inhibition at 10mM | Streptomyces sp. TN119 | |
Cu2+ | 33% inhibition at 10mM | Streptomyces sp. TN119 | |
Hg2+ | complete inhibition at 1 mM | Streptomyces sp. TN119 | |
Mn2+ | 19% inhibition at 5 mM, 60% at 10mM | Streptomyces sp. TN119 | |
additional information | no or poor inhibition by Ni2+, Cr3+, Mg2+, Li+, Fe3+, K+, Na+, Ca2+, and EDTA | Streptomyces sp. TN119 | |
Pb2+ | 18% inhibition at 10mM | Streptomyces sp. TN119 | |
SDS | 84% inhibition at 5 mM, complete at 10mM | Streptomyces sp. TN119 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no or poor inhibition by Ni2+, Cr3+, Mg2+, Li+, Fe3+, K+, Na+, Ca2+, and EDTA | Streptomyces sp. TN119 | |
Zn2+ | activates 1.2fold at 10 mM | Streptomyces sp. TN119 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
x * 38000, recombinant enzyme, SDS-PAGE | Streptomyces sp. TN119 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
oat-spelt xylan + H2O | Streptomyces sp. TN119 | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. TN119 | C6FX34 | isolated from the gut of Batocera horsfieldi larvae collected from oak trees, gene xynA119 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) culture supernatant to homogeneity by nickel affinity chromatography | Streptomyces sp. TN119 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
16.4 | - |
recombinant enzyme, culture supernatant | Streptomyces sp. TN119 |
57.9 | - |
purified recombinant enzyme, substrate oat-spelt xylan | Streptomyces sp. TN119 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
oat-spelt xylan + H2O | - |
Streptomyces sp. TN119 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 38000, recombinant enzyme, SDS-PAGE | Streptomyces sp. TN119 |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme belongs to the glycosyl hydrolases family 10, GH 10 | Streptomyces sp. TN119 |
XynA119 | - |
Streptomyces sp. TN119 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
recombinant enzyme | Streptomyces sp. TN119 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
purified recombinant enzyme, 1 h, 95% remaining activity, rapid decrease in activity above 60°C | Streptomyces sp. TN119 |
65 | - |
purified recombinant enzyme, 1 h, 70% remaining activity | Streptomyces sp. TN119 |
70 | - |
purified recombinant enzyme, 1 h, 45% remaining activity | Streptomyces sp. TN119 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
49.2 | - |
oat-spelt xylan | pH 6.5, 60°C | Streptomyces sp. TN119 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
recombinant enzyme | Streptomyces sp. TN119 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7.5 | over 60% of maximal activity within this range, recombinant enzyme | Streptomyces sp. TN119 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | purified recombinant enzyme, 1 h, 37°C, 85% remaining activity | Streptomyces sp. TN119 |