Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | xylose induces, is the best carbon source. Lactose is the best inducer, sucrose induces, glucose produces low levels of xylanase | Thermochaetoides thermophila |
Application | Comment | Organism |
---|---|---|
industry | is thermostable in alkaline buffer favoring its suitability to bio-bleaching of kraft pulp. Xylanases that are more active in pH range of 6.0-10.0 can act as promising agents in paper and pulp industry. Productivity profiles of xylanase in Escherichia coli recombinant are more than 4fold of that produced from Trichoderma reesei RUTC-30, 5fold of that produced by the donor and significantly higher than the values reported on other Escherichia coli, and Saccharomyces cerevisiae recombinants | Thermochaetoides thermophila |
Cloned (Comment) | Organism |
---|---|
specific and exact size fragment cloned into vector pTZ57R for transformation into Escherichia coli DH5alpha. Cloned xyn11A gene excised from pTZ57R with HindIII and EcoRI and ligated into pET-32a (+) and expressed in Escherichia coli BL21 under T7 promoter | Thermochaetoides thermophila |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | removal of fusion protein rather decreases activity | Thermochaetoides thermophila |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29300 | - |
1 * 29300, sequence analysis, 1 * 30000, SDS-PAGE | Thermochaetoides thermophila |
30000 | - |
1 * 29300, sequence analysis, 1 * 30000, SDS-PAGE | Thermochaetoides thermophila |
43000 | - |
fused xyn11A protein, Western blot analysis | Thermochaetoides thermophila |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermochaetoides thermophila | Q6UN40 | - |
- |
Thermochaetoides thermophila NIBGE 1 | Q6UN40 | - |
- |
Purification (Comment) | Organism |
---|---|
enterokinase-treated enzyme purified by a combination of ammonium sulfate precipitation, gel filtration and ion exchange chromatography, to homogeneity | Thermochaetoides thermophila |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
32.6 | - |
enterokinase-digested endoxylanase | Thermochaetoides thermophila |
38.1 | - |
untreated endoxylanase | Thermochaetoides thermophila |
125 | - |
purified enterokinase-digested endoxylanase | Thermochaetoides thermophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | recombinant Xyn11A fails to hydrolyze CMC, avicel or p-nitrophenyl beta-xylopyranoside | Thermochaetoides thermophila | ? | - |
? | |
additional information | recombinant Xyn11A fails to hydrolyze CMC, avicel or p-nitrophenyl beta-xylopyranoside | Thermochaetoides thermophila NIBGE 1 | ? | - |
? | |
oat spelt xylan + H2O | - |
Thermochaetoides thermophila | D-xylose + xylobiose + ? | - |
? | |
oat spelt xylan + H2O | - |
Thermochaetoides thermophila NIBGE 1 | D-xylose + xylobiose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 29300, sequence analysis, 1 * 30000, SDS-PAGE | Thermochaetoides thermophila |
Synonyms | Comment | Organism |
---|---|---|
endoxylanase | - |
Thermochaetoides thermophila |
xylanase | - |
Thermochaetoides thermophila |
xylanase 11 A | - |
Thermochaetoides thermophila |
Xyn11A | - |
Thermochaetoides thermophila |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Thermochaetoides thermophila |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | - |
Thermochaetoides thermophila |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
retains 35% enzyme activity. The enzyme shows half lives of 1100, 330, 86, 70, 19.3 and 11.4 min at 30, 40, 50, 60, 70 and 80°C, respectively | Thermochaetoides thermophila |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Thermochaetoides thermophila |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | recombinant enzyme | Thermochaetoides thermophila |