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Literature summary for 3.2.1.8 extracted from

  • Jalal, A.; Rashid, N.; Rasool, N.; Akhtar, M.
    Gene cloning and characterization of a xylanase from a newly isolated Bacillus subtilis strain R5 (2009), J. Biosci. Bioeng., 107, 360-365.
    View publication on PubMed

Application

Application Comment Organism
industry the broad temperature profile of the enzyme makes it a potential candidate for its use in the pulp and paper industry Bacillus subtilis

Cloned(Commentary)

Cloned (Comment) Organism
expressed from vector pT-xyl in Escherichia coli strain BL21(DE3)CodonPlus-RIL Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 2fold increase in activity with 0.1 mM CaCl2 Bacillus subtilis
Cd2+ 2fold increase in activity with 0.1 mM CdCl2 Bacillus subtilis
Co2+ 3fold increase in activity with 0.1 mM CoCl2 Bacillus subtilis
Mg2+ 2fold increase in activity with 0.1 mM CoCl2 Bacillus subtilis
additional information activity is not dependent on metal ions Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23350
-
sequence analysis Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis B9ZZN9
-
-
Bacillus subtilis R5 B9ZZN9
-
-

Purification (Commentary)

Purification (Comment) Organism
by ammonium sulfate precipitation, anion-exchange and hydrophobic interaction column chromatographies, 17.5fold purified Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beechwood xylan + H2O low affinity of the enzyme towards this substrate Bacillus subtilis ?
-
?
beechwood xylan + H2O low affinity of the enzyme towards this substrate Bacillus subtilis R5 ?
-
?

Synonyms

Synonyms Comment Organism
xylanase
-
Bacillus subtilis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 50 activity sharply decreases below 30°C and above 60°C Bacillus subtilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
the half-life of the recombinant protein at 50°C is 65 min whereas it is approximately 25 min at 60°C. The enzyme is not stable at 70°C and it loses almost all of its activity even 5 min after incubation Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Bacillus subtilis

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8.5 more than 50% activity retained, enzyme activity decreases sharply below pH 5.5 and above pH 9.0, negligible amount of activity at pH below 5.0 and above 10.5 Bacillus subtilis

pI Value

Organism Comment pI Value Maximum pI Value
Bacillus subtilis
-
-
9.44