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Literature summary for 3.2.1.8 extracted from
- Purification and characterization of a thermostable xylanase from the brown-rot fungus Laetiporus sulphureus (2009), J. Biosci. Bioeng., 107, 33-37.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | in the presence of 10 mM, the relative xylanase activity decreases by 5% | Laetiporus sulphureus |  |
| Cu2+ | in the presence of 10 mM, the relative xylanase activity decreases by 58.1% | Laetiporus sulphureus | |
| EDTA | in the presence of 10 mM, the relative xylanase activity decreases by 12.6% | Laetiporus sulphureus | |
| Fe2+ | in the presence of 10 mM, the relative xylanase activity decreases by 21.1% | Laetiporus sulphureus | |
| Mg2+ | in the presence of 10 mM, the relative xylanase activity decreases by 19.1% | Laetiporus sulphureus | |
| Mn2+ | in the presence of 10 mM, the relative xylanase activity decreases by 44.3% | Laetiporus sulphureus | |
| Zn2+ | in the presence of 10 mM, the relative xylanase activity decreases by 9.2% | Laetiporus sulphureus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | in the presence of 5 mM, the relative xylanase activity increases by 35.9% | Laetiporus sulphureus | |
| potassium acetate | in the presence of 5 mM, the relative xylanase activity increases by 5.8% | Laetiporus sulphureus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 69300 | - |
1 * 70000, SDS-PAGE, 1 * 69300, LabChip microfluidic system | Laetiporus sulphureus |
| 70000 | - |
1 * 70000, SDS-PAGE, 1 * 69300, LabChip microfluidic system | Laetiporus sulphureus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Laetiporus sulphureus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| three consecutive purification steps, gel filtration, 96% purity | Laetiporus sulphureus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.9 | - |
crude filtrate | Laetiporus sulphureus |
| 72.4 | - |
purified enzyme | Laetiporus sulphureus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| avicel + H2O | 10% activity as compared to beechwood xylan | Laetiporus sulphureus | ? | - |
? | |
| beechwood xylan + H2O | highest activity | Laetiporus sulphureus | ? | - |
? | |
| birchwood xylan + H2O | slightly lower activity than with beechwood xylan | Laetiporus sulphureus | xylobiose + xylotetraose + ? | - |
? | |
| carboxylmethyl cellulose + H2O | 10% activity as compared to beechwood xylan | Laetiporus sulphureus | ? | - |
? | |
| additional information | does not hydrolyze xylobiose | Laetiporus sulphureus | ? | - |
? | |
| oat spelt xylan + H2O | 22.3% activity as compared to beechwood xylan | Laetiporus sulphureus | ? | - |
? | |
| xylopentaose + H2O | - |
Laetiporus sulphureus | xylobiose + xylotriose + xylotetraose | - |
? | |
| xylotetraose + H2O | - |
Laetiporus sulphureus | 2 xylobiose | - |
? | |
| xylotriose + H2O | - |
Laetiporus sulphureus | xylobiose + D-xylose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 70000, SDS-PAGE, 1 * 69300, LabChip microfluidic system | Laetiporus sulphureus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endoxylanase | - |
Laetiporus sulphureus |
| xylanase | - |
Laetiporus sulphureus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
- |
Laetiporus sulphureus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | 90 | activity decreases rapidly at temperatures over 80°C, but still retains approximately 70% relative activity at 90°C | Laetiporus sulphureus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
retains approximately 97% of its relative activity following incubation for 4 h | Laetiporus sulphureus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
- |
Laetiporus sulphureus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2.5 | 5 | maintains relative activity over 50% | Laetiporus sulphureus |
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