Application | Comment | Organism |
---|---|---|
industry | high production yields of the modular xylanase Xyl30 by solid-state fermentation and its biochemical features make it a good candidate for use in industrial applications | Streptomyces avermitilis |
Cloned (Comment) | Organism |
---|---|
3588-bp BamHI-XbaI fragment containing genes xyl30 and hd30 introduced into the pN702GEM3 plasmid and overexpressed in Streptomyces lividans strain 66 | Streptomyces avermitilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | modular endoxylanase containing a xylan-binding domain, which after processing in the culture supernatant loses the afore mentioned domain and thus its capacity to bind xylan. Sharp decrease (up to 50%) in activity after 15 min of incubation at 70 or 80°C | Streptomyces avermitilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
1 * 42800, SDS-PAGE, 1 * 35000, SDS-PAGE. Heavier protein band (42.8 kDa) is observed after 12 h of culture, while the lighter band (35 kDa) appears 12 h later | Streptomyces avermitilis |
42800 | - |
1 * 42800, SDS-PAGE, 1 * 35000, SDS-PAGE. Heavier protein band (42.8 kDa) is observed after 12 h of culture, while the lighter band (35 kDa) appears 12 h later | Streptomyces avermitilis |
47000 | - |
sequence analysis, xyl30 ORF | Streptomyces avermitilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces avermitilis | Q9X584 | - |
- |
Streptomyces avermitilis CECT 3339 | Q9X584 | - |
- |
Streptomyces lividans | - |
- |
- |
Streptomyces lividans 66 | - |
- |
- |
Thermomyces lanuginosus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
heavier protein band (42.8 kDa) partially purified | Streptomyces avermitilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arabinoxylan + H2O | - |
Thermomyces lanuginosus | ? | - |
? | |
arabinoxylan + H2O | highest production of xylanase activity after approximately 3 days of incubation. Only the heavier protein band (42.8 kDa) binds to the substrate | Streptomyces avermitilis | ? | - |
? | |
arabinoxylan + H2O | highest production of xylanase activity after approximately 3 days of incubation. Only the heavier protein band (42.8 kDa) binds to the substrate | Streptomyces avermitilis CECT 3339 | ? | - |
? | |
rye bran + H2O | low activity with Streptomyces lividans carrying empty vector pN702GEM3 | Streptomyces lividans | ? | - |
? | |
rye bran + H2O | maximum production of xylanase occurs after 144 h of incubation. When waterwashed rye bran is used as substrate, maximum production is reached after 216 h | Streptomyces avermitilis | ? | - |
? | |
rye bran + H2O | maximum production of xylanase occurs after 144 h of incubation. When waterwashed rye bran is used as substrate, maximum production is reached after 216 h | Streptomyces avermitilis CECT 3339 | ? | - |
? | |
rye bran + H2O | low activity with Streptomyces lividans carrying empty vector pN702GEM3 | Streptomyces lividans 66 | ? | - |
? | |
wheat bran + H2O | low activity with strain Streptomyces lividans carrying empty vector pN702GEM3 | Streptomyces lividans | ? | - |
? | |
wheat bran + H2O | when waterwashed wheat bran is used as substrate, maximum production is reached after 216 h | Streptomyces avermitilis | ? | - |
? | |
wheat bran + H2O | when waterwashed wheat bran is used as substrate, maximum production is reached after 216 h | Streptomyces avermitilis CECT 3339 | ? | - |
? | |
wheat bran + H2O | low activity with strain Streptomyces lividans carrying empty vector pN702GEM3 | Streptomyces lividans 66 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | 1 * 42800, SDS-PAGE, 1 * 35000, SDS-PAGE. Heavier protein band (42.8 kDa) is observed after 12 h of culture, while the lighter band (35 kDa) appears 12 h later | Streptomyces avermitilis |
Synonyms | Comment | Organism |
---|---|---|
endoxylanase | - |
Streptomyces lividans |
endoxylanase | - |
Thermomyces lanuginosus |
endoxylanase | - |
Streptomyces avermitilis |
Xyl30 | - |
Streptomyces avermitilis |
xylanase | - |
Streptomyces lividans |
xylanase | - |
Thermomyces lanuginosus |
xylanase | - |
Streptomyces avermitilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
purified heavier protein band (42.8 kDa) | Streptomyces avermitilis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 80 | purified heavier protein band (42.8 kDa), more than 50% of maximum activity | Streptomyces avermitilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
purified heavier protein band (42.8 kDa), retains more than 40% after a 1 h incubation at 70°C. Retains 35% of its activity after a 1 h incubation at 87°C | Streptomyces avermitilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
purified heavier protein band (42.8 kDa) | Streptomyces avermitilis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 9 | purified heavier protein band (42.8 kDa), retains more than 50% of its activity | Streptomyces avermitilis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Streptomyces avermitilis | - |
- |
6.3 |