Application | Comment | Organism |
---|---|---|
additional information | the Bacillus sp. KT12 xylanolytic enzyme is a suitable enzyme for the synthesis of polyphenyl beta-oligoxylosides | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | inhibits hydrolysis activity | Bacillus subtilis | |
Ca2+ | completely inhibits hydrolysis activity | Bacillus subtilis | |
Co2+ | inhibits hydrolysis activity | Bacillus subtilis | |
Cu2+ | inhibits hydrolysis activity | Bacillus subtilis | |
EDTA | completely inhibits hydrolysis activity | Bacillus subtilis | |
Mg2+ | inhibits hydrolysis activity | Bacillus subtilis | |
Mn2+ | inhibits hydrolysis activity | Bacillus subtilis | |
additional information | hydrolytic activity almost entirely inhibits at acidic pH | Bacillus subtilis | |
Zn2+ | completely inhibits hydrolysis activity | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | strongly activates transxylosylation activity | Bacillus subtilis | |
additional information | other metalions than Fe2+ have an insignificant effect on transxylosylation activity | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
1 * 20000, SDS-PAGE and HPLC | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Aspergillus niger IFO 31628 | - |
- |
- |
Bacillus subtilis | Q45VU2 | - |
- |
Bacillus subtilis KT12 | Q45VU2 | - |
- |
Penicillium expansum | - |
- |
- |
Penicillium expansum IFO 8800 | - |
- |
- |
Trichoderma viride | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by ammonium sulfate fractionation, centrifugation and gel filtration, 7.7fold | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methoxyphenol + H2O | weak transxylosylation activity | Bacillus subtilis | ? | - |
? | |
ascorbic acid + H2O | weak transxylosylation activity | Bacillus subtilis | ? | - |
? | |
beech wood xylan + H2O | - |
Aspergillus niger | ? | - |
? | |
beech wood xylan + H2O | - |
Trichoderma viride | ? | - |
? | |
beech wood xylan + H2O | - |
Penicillium expansum | ? | - |
? | |
beech wood xylan + H2O | strong transxylosylation activity | Bacillus subtilis | ? | - |
? | |
beech wood xylan + H2O | - |
Penicillium expansum IFO 8800 | ? | - |
? | |
beech wood xylan + H2O | - |
Aspergillus niger IFO 31628 | ? | - |
? | |
caffeic acid + H2O | weak transxylosylation activity | Bacillus subtilis | ? | - |
? | |
catechin + H2O | strong transxylosylation activity | Bacillus subtilis | ? | - |
? | |
catechol + H2O | strong transxylosylation activity | Bacillus subtilis | ? | - |
? | |
DOPA + H2O | weak transxylosylation activity | Bacillus subtilis | ? | - |
? | |
dopamine + H2O | weak transxylosylation activity | Bacillus subtilis | ? | - |
? | |
fructose + H2O | weak transxylosylation activity | Bacillus subtilis | ? | - |
? | |
gallic acid + H2O | strong transxylosylation activity | Bacillus subtilis | ? | - |
? | |
glucose + H2O | weak transxylosylation activity | Bacillus subtilis | ? | - |
? | |
glucose + H2O | weak transxylosylation activity | Bacillus subtilis KT12 | ? | - |
? | |
hydroquinone + H2O | strong transxylosylation activity | Bacillus subtilis | ? | - |
? | |
lactose + H2O | strong transxylosylation activity | Bacillus subtilis | ? | - |
? | |
lactose + H2O | strong transxylosylation activity | Bacillus subtilis KT12 | ? | - |
? | |
maltose + H2O | strong transxylosylation activity | Bacillus subtilis | ? | - |
? | |
maltose + H2O | strong transxylosylation activity | Bacillus subtilis KT12 | ? | - |
? | |
mannitol + H2O | weak transxylosylation activity | Bacillus subtilis | ? | - |
? | |
additional information | displays no transxylosylation activity toward xylitol, cellulose, chitin, chitosan, pectin, sorbitol, ribose, phenol, guaiacol, o-nitorphenol, vanilin and alcoholic compounds (methanol, ethanol, ethylene grecol and grecerol) | Bacillus subtilis | ? | - |
? | |
additional information | displays no transxylosylation activity toward xylitol, cellulose, chitin, chitosan, pectin, sorbitol, ribose, phenol, guaiacol, o-nitorphenol, vanilin and alcoholic compounds (methanol, ethanol, ethylene grecol and grecerol) | Bacillus subtilis KT12 | ? | - |
? | |
nutrient broth + H2O | strong transxylosylation activity | Bacillus subtilis | ? | - |
? | |
phloroglucinol + H2O | weak transxylosylation activity | Bacillus subtilis | ? | - |
? | |
pyrogallol + H2O | strong transxylosylation activity | Bacillus subtilis | ? | - |
? | |
resorcinol + H2O | weak transxylosylation activity | Bacillus subtilis | ? | - |
? | |
starch + H2O | strong transxylosylation activity | Bacillus subtilis | ? | - |
? | |
sucrose + H2O | strong transxylosylation activity | Bacillus subtilis | D-fructose + D-glucose | - |
? | |
sucrose + H2O | strong transxylosylation activity | Bacillus subtilis KT12 | D-fructose + D-glucose | - |
? | |
xylooligosaccharide + H2O | weak transxylosylation activity | Bacillus subtilis | ? | - |
? | |
xylose + H2O | weak transxylosylation activity | Bacillus subtilis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 20000, SDS-PAGE and HPLC | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
endoxylanase | - |
Aspergillus niger |
endoxylanase | - |
Trichoderma viride |
endoxylanase | - |
Penicillium expansum |
endoxylanase | - |
Bacillus subtilis |
xylanase | - |
Aspergillus niger |
xylanase | - |
Trichoderma viride |
xylanase | - |
Penicillium expansum |
xylanase | - |
Bacillus subtilis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 60 | transxylosylation and hydrolysis activities are optimal at temperatures of 60°C and 40°C, respectively | Bacillus subtilis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 11 | near constant transxylosylation activity | Bacillus subtilis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bacillus subtilis | IEF-PAGE | - |
7.5 |