Literature summary for 3.2.1.8 extracted from

Li, N.; Meng, K.; Wang, Y.; Shi, P.; Luo, H.; Bai, Y.; Yang, P.; Yao, B.
Cloning, expression, and characterization of a new xylanase with broad temperature adaptability from Streptomyces sp. S9 (2008), Appl. Microbiol. Biotechnol., 80, 231-240.

Search for more literature for 3.2.1.8

Activating Compound

Activating Compound	Comment	Organism	Structure
beta-mercaptoethanol	10 mM enhances the activity about 1.6fold	Streptomyces sp. S9
additional information	Na+, Ca2+, Ni2+, Mg2+, Fe3+, Zn2+, Li+, EDTA and sodium dodecyl sulfate have no effect	Streptomyces sp. S9

Application

Application	Comment	Organism
industry	favorable properties of XynAS9, such as high activity over a wide temperature range, good thermal and pH stability, and less complex hydrolysis products, make this xylanase promising for various applications in the animal feed and biofuel industries	Streptomyces sp. S9

Cloned(Commentary)

Cloned (Comment)	Organism
into pET-22b(+) vector and expressed in Escherichia coli BL21 (DE3)	Streptomyces sp. S9

Inhibitors

Inhibitors	Comment	Organism	Structure
Ag+	1 mM strongly inhibits	Streptomyces sp. S9
Co2+	10 mM partialy inhibits	Streptomyces sp. S9
Cu2+	10 mM partialy inhibits	Streptomyces sp. S9
Hg2+	1 mM completely inhibits	Streptomyces sp. S9
K+	10 mM partialy inhibits	Streptomyces sp. S9
Mn2+	10 mM partialy inhibits	Streptomyces sp. S9
additional information	Na+, Ca2+, Ni2+, Mg2+, Fe3+, Zn2+, Li+, EDTA and sodium dodecyl sulfate have no effect	Streptomyces sp. S9
Pb2+	10 mM partialy inhibits	Streptomyces sp. S9

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Cr3+	10 mM enhances the activity about 1.3fold	Streptomyces sp. S9

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
46000	-	1 * 46000, SDS-PAGE	Streptomyces sp. S9

Organism

Organism	UniProt	Comment	Textmining
Streptomyces sp. S9	B4XVN1	-	-

Purification (Commentary)

Purification (Comment)	Organism
to electrophoretic homogeneity by ammonium sulfate precipitation, ion exchange chromatography, and hydrophobic interaction chromatography, 7.1fold purified, with a final activity yield of 18.1%	Streptomyces sp. S9

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
63.6	-	culture supernatant	Streptomyces sp. S9
453.7	-	7.1fold purified enzyme	Streptomyces sp. S9

Storage Stability

Storage Stability	Organism
4°C, 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.5	Streptomyces sp. S9

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
birchwood xylan + H2O	xylose and xylobiose are the predominant products	Streptomyces sp. S9	D-xylose + xylobiose + ?	-	?
oat spelt xylan + H2O	xylose and xylobiose are the predominant products	Streptomyces sp. S9	D-xylose + xylobiose + ?	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 46000, SDS-PAGE	Streptomyces sp. S9

Synonyms

Synonyms	Comment	Organism
xylanase	-	Streptomyces sp. S9
XynAS9	-	Streptomyces sp. S9

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
60	-	-	Streptomyces sp. S9

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
50	80	retains more than 65% of the maximum activity	Streptomyces sp. S9

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	-	Streptomyces sp. S9

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Release 2023.1 (January 2023)