Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | in the insoluble fraction of cell lysate or embedding in artificial oil bodies, XynS20-intein M-oleosin does not show any enzymatic activity. After release from artificial oil bodies by inclusion of DTT to induce intein linker self-splicing, XynS20 reveals a xylanase activity band of about 36 kDa | Neocallimastix patriciarum |
Cloned (Comment) | Organism |
---|---|
DNA fragments of xynS20 genes subcloned into vector pOSP2. XynS20 expressed as a recombinant protein fused to the N-terminus of oleosin by a linker polypeptide, intein M, in Escherichia coli | Neocallimastix patriciarum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
sequence analysis | Neocallimastix patriciarum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neocallimastix patriciarum | A8TGA1 | - |
- |
Purification (Comment) | Organism |
---|---|
affinity-purified by formation of artificial oil bodies | Neocallimastix patriciarum |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1983 | - |
toward oat spelt xylan | Neocallimastix patriciarum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity against carboxy methyl cellulose, beta-glucan, lichenan, laminarin, agarose, starch, and kappa-carageenan | Neocallimastix patriciarum | ? | - |
? | |
oat spelt xylan + H2O | - |
Neocallimastix patriciarum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
xylanase | - |
Neocallimastix patriciarum |
XynS20 | - |
Neocallimastix patriciarum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Neocallimastix patriciarum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 55 | at temperatures below 30°C and above 55°C, enzyme activity is less than 50% of that at the optimal temperature | Neocallimastix patriciarum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Neocallimastix patriciarum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 6 | - |
Neocallimastix patriciarum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 7 | when pH is below 4.0 or above 7.0, less than 60% of the optimal activity is retained and the enzyme shows no activity at pH 9.0 | Neocallimastix patriciarum |