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Literature summary for 3.2.1.8 extracted from

  • Li, Y.; Liu, Z.; Cui, F.; Ping, L.; Qiu, C.; Li, G.; Yan, L.
    Isolation and identification of a newly isolated Alternaria sp. ND-16 and characterization of xylanase (2009), Appl. Biochem. Biotechnol., 157, 36-49.
    View publication on PubMed
Search for more literature for 3.2.1.8

Activating Compound

Activating Compound Comment Organism Structure
(NH4)2SO4 xylanase activity is 9.7 U/ml with 0.1% urea Alternaria mali
Ca2+ slightly stimulates Alternaria mali
casein hydrolysate xylanase activity is 5.3 U/ml with 0.1% urea Alternaria mali
Co2+ slightly stimulates Alternaria mali
Mg2+ slightly stimulates Alternaria mali
NaNO3 xylanase activity is 11.9 U/ml with 0.1% urea Alternaria mali
NH4Cl xylanase activity is 10.5 U/ml with 0.1% urea Alternaria mali
tryptone xylanase activity is 6.5 U/ml with 0.1% urea Alternaria mali
Urea maximum xylanase activity (14.2 U/ml) is evident with 0.1% urea Alternaria mali
yeast extract xylanase activity is 6.2 U/ml with 0.1% urea Alternaria mali

Application

Application Comment Organism
industry the suitable temperature range for industrial application of xylanase from Alternaria mali ND-16 is 50-55°C Alternaria mali

Inhibitors

Inhibitors Comment Organism Structure
Mn2+ strongly inhibits 80.5% of xylanase activity Alternaria mali
additional information K+, Li+, Fe2+, Cu2+, and Zn2+ have no significant effect on activity. Slight decrease in xylanase production for both oat spelt xylan medium and wheat bran medium when prolonging the cultivation time Alternaria mali
Na+ strongly inhibits 33.2% of xylanase activity Alternaria mali

Organism

Organism UniProt Comment Textmining
Alternaria mali
-
-
-
Alternaria mali ND-16
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partially purified by ammonium sulphate fractionation Alternaria mali

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with starch, glucose, fructose, sorbitol, or maltose Alternaria mali ?
-
 ?
additional information no activity with starch, glucose, fructose, sorbitol, or maltose Alternaria mali ND-16 ?
-
 ?
oat spelt xylan + H2O xylanase production only reaches 5.7 and 11.7 U/ml when using pure xylan (oat spelt xylan) as the carbon source at 0.5% and 1% level, respectively Alternaria mali ?
-
 ?
oat spelt xylan + H2O xylanase production only reaches 5.7 and 11.7 U/ml when using pure xylan (oat spelt xylan) as the carbon source at 0.5% and 1% level, respectively Alternaria mali ND-16 ?
-
 ?
wheat bran + H2O xylanase production is highest (19.1 U/ml) when 1% wheat bran is added as the carbon source Alternaria mali ?
-
 ?
wheat bran + H2O xylanase production is highest (19.1 U/ml) when 1% wheat bran is added as the carbon source Alternaria mali ND-16 ?
-
 ?

Synonyms

Synonyms Comment Organism
xylanase
-
 Alternaria mali

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Alternaria mali

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 55
-
 Alternaria mali

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45 55 slight decrease in xylanase activity between 45°C (93.4%) and 55°C (97.7%). Enzyme activity is rapidly reduced to 59.3% when the temperature is at 60°C, and there is negligible activity (5.4%) above 80°C Alternaria mali

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Alternaria mali

pH Range

pH Minimum pH Maximum Comment Organism
5.8 6.2
-
 Alternaria mali