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Literature summary for 3.2.1.8 extracted from

  • Sun, J.Y.; Liu, M.Q.; Weng, X.Y.
    Hydrolytic properties of a hybrid xylanase and its parents (2009), Appl. Biochem. Biotechnol., 152, 428-439.
    View publication on PubMed

Application

Application Comment Organism
additional information the hybrid xylanase, whose parents are Thermomonospora fusca xylanase A and Aspergillus niger xylanase A, inherits some hydrolytic properties from its parents, and it is an endo-acting xylanase. X4 may be the minimum oligomer hydrolyzed by it Aspergillus niger
additional information the hybrid xylanase, whose parents are Thermomonospora fusca xylanase A and Aspergillus niger xylanase A, inherits some hydrolytic properties from its parents, and it is an endo-acting xylanase. X4 may be the minimum oligomer hydrolyzed by it Thermobifida fusca

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-
Thermobifida fusca
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Aspergillus niger
-
Thermobifida fusca

Storage Stability

Storage Stability Organism
4°C Aspergillus niger
4°C Thermobifida fusca

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
birchwood xylan + H2O can be hydrolyzed by hybrid xylanase and its Aspergillus parent. Hydrolysis products by hybrid xylanase and its Aspergillus parent are D-xylose, xylobiose, xylotriose, xylotetraose and xylopentaose. Xylotriose is the main product released from birchwood xylan and wheat bran insoluble xylan by hybrid xylanase and its Aspergillus parent, respectively Aspergillus niger D-xylose + xylobiose + xylotriose + xylotetraose + xylopentaose + xylohexaose
-
?
birchwood xylan + H2O can be hydrolyzed by hybrid xylanase and its Thermomonospora parent. Hydrolysis products by hybrid xylanase are D-xylose, xylobiose, xylotriose, xylotetraose and xylopentaose and by its Thermomonospora parent are D-xylose, xylobiose, xylotriose, xylotetraose, xylopentaose and xylohexaose. Xylotriose is the main product released from birchwood xylan and wheat bran insoluble xylan by hybrid xylanase. Xylobiose is the main product separately released from birchwood xylan and wheat bran insoluble xylan by the Thermomonospora parent Thermobifida fusca D-xylose + xylobiose + xylotriose + xylotetraose + xylopentaose + xylohexaose
-
?
wheat bran xylan + H2O can be hydrolyzed by hybrid xylanase and its Aspergillus parent. Hydrolysis products by hybrid xylanase and its Aspergillus parent are D-xylose, xylobiose, xylotriose, xylotetraose, xylopentaose and xylohexaose. Xylotriose is the main product released from birchwood xylan and wheat bran insoluble xylan by hybrid xylanase and its Aspergillus parent, respectively Aspergillus niger D-xylose + xylobiose + xylotriose + xylotetraose + xylopentaose + xylohexaose
-
?
wheat bran xylan + H2O can be hydrolyzed by hybrid xylanase and its Thermomonospora parent. Hydrolysis products by hybrid xylanase and its Thermomonospora parent are D-xylose, xylobiose, xylotriose, xylotetraose, xylopentaose and xylohexaose. Xylotriose is the main product released from birchwood xylan and wheat bran insoluble xylan by hybrid xylanase. Xylobiose is the main product separately released from birchwood xylan and wheat bran insoluble xylan by the Thermomonospora parent Thermobifida fusca D-xylose + xylobiose + xylotriose + xylotetraose + xylopentaose + xylohexaose
-
?
xylobiose + H2O can be hydrolyzed by the Aspergillus parent, no activity with hybrid xylanase Aspergillus niger ?
-
?
xylobiose + H2O can be hydrolyzed by the Thermomonospora parent, no activity with hybrid xylanase Thermobifida fusca 2 D-xylose
-
?
xylohexaose + H2O can be hydrolyzed by the Aspergillus parent and hybrid xylanase Aspergillus niger 2 xylotriose
-
?
xylohexaose + H2O can be hydrolyzed by the Thermomonospora parent and hybrid xylanase Thermobifida fusca 2 xylotriose
-
?
xylopentaose + H2O can be hydrolyzed by the Aspergillus parent and hybrid xylanase Aspergillus niger xylobiose + xylotriose + xylotetraose
-
?
xylopentaose + H2O can be hydrolyzed by the Thermomonospora parent and hybrid xylanase Thermobifida fusca xylobiose + xylotriose + xylotetraose
-
?
xylotetraose + H2O can be hydrolyzed by the Aspergillus parent and hybrid xylanase Aspergillus niger 2 xylobiose
-
?
xylotetraose + H2O can be hydrolyzed by the Thermomonospora parent and hybrid xylanase Thermobifida fusca 2 xylobiose
-
?
xylotriose + H2O can be hydrolyzed by the Aspergillus parent, no activity with hybrid xylanase Aspergillus niger ?
-
?
xylotriose + H2O can be hydrolyzed by the Thermomonospora parent, no activity with hybrid xylanase Thermobifida fusca ?
-
?

Synonyms

Synonyms Comment Organism
xylanase A
-
Aspergillus niger
xylanase A
-
Thermobifida fusca

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50 60 optimum temperature for hybrid xylanase and its Aspergillus parent is 60°C and 50°C, respectively Aspergillus niger
60
-
hybrid xylanase and its Thermomonospora parent Thermobifida fusca

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
hybrid xylanase and its Aspergillus parent Aspergillus niger
5 6 optimum pH for hybrid xylanase and its Thermomonospora parent is 5.0 and 6.0, respectively Thermobifida fusca