Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | activity of rtEGXA depends on the concentration of Cl- | Ampullaria crossean |
Cloned (Comment) | Organism |
---|---|
full-length EGXA cloned into pFastBac vector and transformed into bacmid-containing Escherichia coli DH10Bac cells. Heterogeneously expressed in insect Tn5 cells | Ampullaria crossean |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | cellulose-binding domain decreases the recombinant EGXA's specific activities on p-nitrophenyl-beta-D-cellobioside and sodium carboxymethyl cellulose | Ampullaria crossean |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63000 | - |
1 * 63000, SDS-PAGE, recombinant EGXA | Ampullaria crossean |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ampullaria crossean | Q7Z1V6 | - |
- |
Purification (Comment) | Organism |
---|---|
on Ni-NTA resin | Ampullaria crossean |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
stomach | EGX, truncated version of EGXA | Ampullaria crossean | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.9 | - |
recombinant EGXA with p-nitrophenyl beta-D-cellobioside as substrate | Ampullaria crossean |
7.5 | - |
recombinant EGXA with sodium carboxymethyl cellulose as substrate | Ampullaria crossean |
12.5 | - |
wild-type EGX with sodium carboxymethyl cellulose as substrate | Ampullaria crossean |
40.3 | - |
wild-type EGX with p-nitrophenyl beta-D-cellobioside as substrate | Ampullaria crossean |
275 | - |
wild-type EGX with xylan as substrate | Ampullaria crossean |
391.8 | - |
recombinant EGXA with xylan as substrate | Ampullaria crossean |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
p-nitrophenyl beta-D-cellobioside + H2O | - |
Ampullaria crossean | p-nitrophenol + beta-D-cellobiose | - |
? | |
sodium carboxymethyl cellulose + H2O | recombinant EGXA with a cellulose-binding domain binds to the cellulose with higher affinity than wild-type EGX, which lacks the cellulose-binding domain | Ampullaria crossean | ? | - |
? | |
xylan + H2O | - |
Ampullaria crossean | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 63000, SDS-PAGE, recombinant EGXA | Ampullaria crossean |
Synonyms | Comment | Organism |
---|---|---|
EGXA | - |
Ampullaria crossean |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
recombinant EGXA with xylan as substrate | Ampullaria crossean |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
recombinant EGXA and wild-type EGX lose activity quickly when Cl- is at a low concentration. In the presence of 50 mM Cl-, the rtEGXAs activity loss is only 15% after 5 h incubation at 45°C. Wild-type EGX loses activity at a faster speed than the recombinant EGXA, in both 0.5 mM Cl- and 50 mM Cl-. Cellulose-binding domain increases thermal stability of the recombinant enzyme | Ampullaria crossean |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
recombinant EGXA with xylan as substrate | Ampullaria crossean |