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Literature summary for 3.2.1.8 extracted from
- Characterization of xylanase from Lentinus edodes M290 cultured on waste mushroom logs (2007), J. Microbiol. Biotechnol., 17, 1811-1817.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
SDS-PAGE | Lentinula edodes |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lentinula edodes | - |
- |
- |
| Lentinula edodes M290 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Mono Q column chromatography and Superdex 75 gel filtration | Lentinula edodes |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.45 | - |
crude culture, in 50 mM sodium acetate buffer (pH 4.5) at 30°C | Lentinula edodes |
| 90.38 | - |
after purification, in 50 mM sodium acetate buffer (pH 4.5) at 30°C | Lentinula edodes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| xylan + H2O | - |
Lentinula edodes | ? | - |
? |  |
| xylan + H2O | - |
Lentinula edodes M290 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| xylanase | - |
Lentinula edodes |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Lentinula edodes |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 60 | more than 50% of the optimal xylanase activity is retained when the temperature is increased from 20 to 60°C after 240 min, at 40°C the xylanase maintains 93% of the optimal activity after a 240 min reaction, the enzyme retains 78.12% and 41.64% of the maximum activity at 50 and 60°C, respectively. at temperatures up to 60°C, xylanase activity decreases sharply with time | Lentinula edodes |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
- |
Lentinula edodes |
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Release 2023.1 (January 2023)
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