Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Saccharolobus solfataricus | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55500 | - |
SDS-PAGE | Saccharolobus solfataricus |
58800 | - |
gel filtration | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Saccharolobus solfataricus MT-4 / DSM 5833 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Superdex 200 HR 10/30 gel filtration and Mono Q HR 5/5 column chromatography | Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.1 | - |
crude extract, using oat spelt xylan as a substrate, at pH 7.0, at 80°C | Saccharolobus solfataricus |
11.4 | - |
after 10.4fold purification, using oat spelt xylan as a substrate, at pH 7.0, at 80°C | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
avicel + H2O | weak reaction | Saccharolobus solfataricus | ? | - |
? | |
avicel + H2O | weak reaction | Saccharolobus solfataricus MT-4 / DSM 5833 | ? | - |
? | |
beechwood xylan + H2O | 100% activity | Saccharolobus solfataricus | ? | - |
? | |
beechwood xylan + H2O | 100% activity | Saccharolobus solfataricus MT-4 / DSM 5833 | ? | - |
? | |
birchwood xylan + H2O | 67% activity compared to beechwood xylan | Saccharolobus solfataricus | ? | - |
? | |
birchwood xylan + H2O | 67% activity compared to beechwood xylan | Saccharolobus solfataricus MT-4 / DSM 5833 | ? | - |
? | |
oat spelt xylan + H2O | 87% activity compared to beechwood xylan | Saccharolobus solfataricus | additional information | xylooligosaccharides are the enzymatic products of xylan hydrolysis, the smallest degradation product is xylobiose | ? | |
oat spelt xylan + H2O | 87% activity compared to beechwood xylan | Saccharolobus solfataricus MT-4 / DSM 5833 | additional information | xylooligosaccharides are the enzymatic products of xylan hydrolysis, the smallest degradation product is xylobiose | ? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 58800, gel filtration | Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
endoxylanase | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
- |
Saccharolobus solfataricus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 100 | - |
Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
50% maximal activity is measured after 85 min at 90°C, 23% residual activity after 3 h at 90°C | Saccharolobus solfataricus |
100 | - |
half-life of 47 min at 100°C | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
at 80°C | Saccharolobus solfataricus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | - |
shows 46% maximal activity at pH 4.0 | Saccharolobus solfataricus |
8 | - |
retains 63% activity at pH 8.0 | Saccharolobus solfataricus |
9 | - |
retains 40% activity at pH 9.0 | Saccharolobus solfataricus |