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Literature summary for 3.2.1.8 extracted from

  • Cannio, R.; Di Prizito, N.; Rossi, M.; Morana, A.
    A xylan-degrading strain of Sulfolobus solfataricus: isolation and characterization of the xylanase activity (2004), Extremophiles, 8, 117-124.
    View publication on PubMed

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Saccharolobus solfataricus 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55500
-
SDS-PAGE Saccharolobus solfataricus
58800
-
gel filtration Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-
Saccharolobus solfataricus MT-4 / DSM 5833
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Superdex 200 HR 10/30 gel filtration and Mono Q HR 5/5 column chromatography Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1
-
crude extract, using oat spelt xylan as a substrate, at pH 7.0, at 80°C Saccharolobus solfataricus
11.4
-
after 10.4fold purification, using oat spelt xylan as a substrate, at pH 7.0, at 80°C Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
avicel + H2O weak reaction Saccharolobus solfataricus ?
-
?
avicel + H2O weak reaction Saccharolobus solfataricus MT-4 / DSM 5833 ?
-
?
beechwood xylan + H2O 100% activity Saccharolobus solfataricus ?
-
?
beechwood xylan + H2O 100% activity Saccharolobus solfataricus MT-4 / DSM 5833 ?
-
?
birchwood xylan + H2O 67% activity compared to beechwood xylan Saccharolobus solfataricus ?
-
?
birchwood xylan + H2O 67% activity compared to beechwood xylan Saccharolobus solfataricus MT-4 / DSM 5833 ?
-
?
oat spelt xylan + H2O 87% activity compared to beechwood xylan Saccharolobus solfataricus additional information xylooligosaccharides are the enzymatic products of xylan hydrolysis, the smallest degradation product is xylobiose ?
oat spelt xylan + H2O 87% activity compared to beechwood xylan Saccharolobus solfataricus MT-4 / DSM 5833 additional information xylooligosaccharides are the enzymatic products of xylan hydrolysis, the smallest degradation product is xylobiose ?

Subunits

Subunits Comment Organism
monomer 1 * 58800, gel filtration Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
endoxylanase
-
Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
-
Saccharolobus solfataricus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60 100
-
Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
50% maximal activity is measured after 85 min at 90°C, 23% residual activity after 3 h at 90°C Saccharolobus solfataricus
100
-
half-life of 47 min at 100°C Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
at 80°C Saccharolobus solfataricus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
shows 46% maximal activity at pH 4.0 Saccharolobus solfataricus
8
-
retains 63% activity at pH 8.0 Saccharolobus solfataricus
9
-
retains 40% activity at pH 9.0 Saccharolobus solfataricus