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Literature summary for 3.2.1.8 extracted from

  • Kalogeris, E.; Christakopoulos, P.; Vrsanska, M.; Kekos, D.; Biely, P.; Macris, B.J.
    Catalytic properties of the endoxylanase I from Thermoascus aurantiacus (2001), J. Mol. Catal. B, 11, 491-501.
No PubMed abstract available
Search for more literature for 3.2.1.8

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.067
-
 4-methylumbelliferyl-xylotriose pH 5.0, 50°C Thermoascus aurantiacus
0.167
-
 4-methylumbelliferyl-xylobiose pH 5.0, 50°C Thermoascus aurantiacus
59.4
-
 4-nitrophenyl alpha-L-arabinopyranoside pH 5.0, 50°C Thermoascus aurantiacus
65.6
-
 4-nitrophenyl alpha-L-arabinofuranoside pH 5.0, 50°C Thermoascus aurantiacus
82
-
 4-nitrophenyl beta-D-cellobioside pH 5.0, 50°C Thermoascus aurantiacus
297
-
 4-nitrophenyl beta-D-xylopyranoside pH 5.0, 50°C Thermoascus aurantiacus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
 gel filtration Thermoascus aurantiacus
33000
-
 1 * 33000, SDS-PAGE Thermoascus aurantiacus

Organism

Organism UniProt Comment Textmining
Thermoascus aurantiacus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermoascus aurantiacus

Reaction

Reaction Comment Organism Reaction ID
(Xylbeta(1-4))n + H2O = (Xylbeta(1-4))n-m + (Xylbeta(1-4))m mode of attack on substrates Thermoascus aurantiacus
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Thermoascus aurantiacus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-xylobiose + H2O hydrolysis at the glycosidic linkage Thermoascus aurantiacus ?
-
 ?
4-methylumbelliferyl-xylotriose + H2O hydrolysis at the glycosidic linkage Thermoascus aurantiacus ?
-
 ?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Thermoascus aurantiacus 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
4-nitrophenyl alpha-L-arabinopyranoside + H2O
-
 Thermoascus aurantiacus 4-nitrophenol + alpha-L-arabinopyranose
-
 ?
4-nitrophenyl beta-D-cellobioside + H2O
-
 Thermoascus aurantiacus 4-nitrophenol + beta-D-cellobiose
-
 ?
4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Thermoascus aurantiacus 4-nitrophenol + beta-D-xylopyranose
-
 ?
arabinoxylan + H2O
-
 Thermoascus aurantiacus arabinose + xylose + xylobiose
-
 ?
glucuronoxylan + H2O
-
 Thermoascus aurantiacus MeGlcA-alpha(1-2)-Xyl-beta(1-4)-Xyl-beta(1-4)-Xyl + ? shortest acidic fragment ?
additional information enzyme tolerates the replacement of beta-xylopyranosyl units in several artificial substrates by beta-glucopyranosyl, alpha-L-arabinopyranosyl, and alpha-L-arabinofuranosyl units Thermoascus aurantiacus ?
-
 ?
O-acetyl-4-O-methyl-glucuronoxylan + H2O
-
 Thermoascus aurantiacus short-acetylated and non-acetylated fragments of 4-O-methyl-glucuronoxylan
-
 ?
rhodymenan + H2O
-
 Thermoascus aurantiacus Xyl-beta(1-3)-Xyl-beta(1-4)-Xyl + ? isomeric xylotriose ?
xylohexaose + H2O
-
 Thermoascus aurantiacus ?
-
 ?
xylopentaose + H2O
-
 Thermoascus aurantiacus ?
-
 ?
xylotetraose + H2O
-
 Thermoascus aurantiacus ?
-
 ?
xylotriose + H2O
-
 Thermoascus aurantiacus ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 33000, SDS-PAGE Thermoascus aurantiacus

Synonyms

Synonyms Comment Organism
endoxylanase I
-
 Thermoascus aurantiacus
More enzyme belongs to family 10 Thermoascus aurantiacus
XYL1
-
 Thermoascus aurantiacus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 assay at Thermoascus aurantiacus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
85.5
-
 4-nitrophenyl alpha-L-arabinofuranoside pH 5.0, 50°C Thermoascus aurantiacus
133.3
-
 4-nitrophenyl alpha-L-arabinopyranoside pH 5.0, 50°C Thermoascus aurantiacus
769
-
 4-nitrophenyl beta-D-xylopyranoside pH 5.0, 50°C Thermoascus aurantiacus
833
-
 4-methylumbelliferyl-xylotriose pH 5.0, 50°C Thermoascus aurantiacus
3333
-
 4-methylumbelliferyl-xylobiose pH 5.0, 50°C Thermoascus aurantiacus
5000
-
 4-nitrophenyl beta-D-cellobioside pH 5.0, 50°C Thermoascus aurantiacus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 assay at Thermoascus aurantiacus