KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.067 | - |
4-methylumbelliferyl-xylotriose | pH 5.0, 50°C | Thermoascus aurantiacus | |
0.167 | - |
4-methylumbelliferyl-xylobiose | pH 5.0, 50°C | Thermoascus aurantiacus | |
59.4 | - |
4-nitrophenyl alpha-L-arabinopyranoside | pH 5.0, 50°C | Thermoascus aurantiacus | |
65.6 | - |
4-nitrophenyl alpha-L-arabinofuranoside | pH 5.0, 50°C | Thermoascus aurantiacus | |
82 | - |
4-nitrophenyl beta-D-cellobioside | pH 5.0, 50°C | Thermoascus aurantiacus | |
297 | - |
4-nitrophenyl beta-D-xylopyranoside | pH 5.0, 50°C | Thermoascus aurantiacus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
gel filtration | Thermoascus aurantiacus |
33000 | - |
1 * 33000, SDS-PAGE | Thermoascus aurantiacus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoascus aurantiacus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermoascus aurantiacus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(Xylbeta(1-4))n + H2O = (Xylbeta(1-4))n-m + (Xylbeta(1-4))m | mode of attack on substrates | Thermoascus aurantiacus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Thermoascus aurantiacus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl-xylobiose + H2O | hydrolysis at the glycosidic linkage | Thermoascus aurantiacus | ? | - |
? | |
4-methylumbelliferyl-xylotriose + H2O | hydrolysis at the glycosidic linkage | Thermoascus aurantiacus | ? | - |
? | |
4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Thermoascus aurantiacus | 4-nitrophenol + alpha-L-arabinofuranose | - |
? | |
4-nitrophenyl alpha-L-arabinopyranoside + H2O | - |
Thermoascus aurantiacus | 4-nitrophenol + alpha-L-arabinopyranose | - |
? | |
4-nitrophenyl beta-D-cellobioside + H2O | - |
Thermoascus aurantiacus | 4-nitrophenol + beta-D-cellobiose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Thermoascus aurantiacus | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
arabinoxylan + H2O | - |
Thermoascus aurantiacus | arabinose + xylose + xylobiose | - |
? | |
glucuronoxylan + H2O | - |
Thermoascus aurantiacus | MeGlcA-alpha(1-2)-Xyl-beta(1-4)-Xyl-beta(1-4)-Xyl + ? | shortest acidic fragment | ? | |
additional information | enzyme tolerates the replacement of beta-xylopyranosyl units in several artificial substrates by beta-glucopyranosyl, alpha-L-arabinopyranosyl, and alpha-L-arabinofuranosyl units | Thermoascus aurantiacus | ? | - |
? | |
O-acetyl-4-O-methyl-glucuronoxylan + H2O | - |
Thermoascus aurantiacus | short-acetylated and non-acetylated fragments of 4-O-methyl-glucuronoxylan | - |
? | |
rhodymenan + H2O | - |
Thermoascus aurantiacus | Xyl-beta(1-3)-Xyl-beta(1-4)-Xyl + ? | isomeric xylotriose | ? | |
xylohexaose + H2O | - |
Thermoascus aurantiacus | ? | - |
? | |
xylopentaose + H2O | - |
Thermoascus aurantiacus | ? | - |
? | |
xylotetraose + H2O | - |
Thermoascus aurantiacus | ? | - |
? | |
xylotriose + H2O | - |
Thermoascus aurantiacus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 33000, SDS-PAGE | Thermoascus aurantiacus |
Synonyms | Comment | Organism |
---|---|---|
endoxylanase I | - |
Thermoascus aurantiacus |
More | enzyme belongs to family 10 | Thermoascus aurantiacus |
XYL1 | - |
Thermoascus aurantiacus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
assay at | Thermoascus aurantiacus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
85.5 | - |
4-nitrophenyl alpha-L-arabinofuranoside | pH 5.0, 50°C | Thermoascus aurantiacus | |
133.3 | - |
4-nitrophenyl alpha-L-arabinopyranoside | pH 5.0, 50°C | Thermoascus aurantiacus | |
769 | - |
4-nitrophenyl beta-D-xylopyranoside | pH 5.0, 50°C | Thermoascus aurantiacus | |
833 | - |
4-methylumbelliferyl-xylotriose | pH 5.0, 50°C | Thermoascus aurantiacus | |
3333 | - |
4-methylumbelliferyl-xylobiose | pH 5.0, 50°C | Thermoascus aurantiacus | |
5000 | - |
4-nitrophenyl beta-D-cellobioside | pH 5.0, 50°C | Thermoascus aurantiacus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
assay at | Thermoascus aurantiacus |