Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli XL1-Blue | Trichoderma reesei |
Protein Variants | Comment | Organism |
---|---|---|
additional information | increase in thermal stability in the mutants leads to increased pH stability as well, but also to reduced activity at both acidic and alkaline pH levels | Trichoderma reesei |
N11D | site-directed mutagenesis, increase of half-life to about 100 min at 65°C | Trichoderma reesei |
N38E | site-directed mutagenesis, increase of half-life to about 100 min at 65°C | Trichoderma reesei |
Q162H | site-directed mutagenesis, mutation at the C-terminus of the alpha-helix has a stabilizing effect at 55°C, not at 65°C | Trichoderma reesei |
Q162Y | site-directed mutagenesis, mutation at the C-terminus of the alpha-helix has a stabilizing effect at 55°C, not at 65°C | Trichoderma reesei |
S110C/N154C | site-directed mutagenesis, introduction of a disulfide bridge in the alpha-helix of the enzyme leads to increase of the half-life at 65°C from less than 1 min to 14 min | Trichoderma reesei |
S110C/N154C/Q162H | site-directed mutagenesis, mutations lead to increased thermal and pH stability, overview | Trichoderma reesei |
S110C/N154C/Q162H/N11D | site-directed mutagenesis, mutations lead to increased thermal and pH stability, overview | Trichoderma reesei |
S110C/N154C/Q162H/N11D/N38D | site-directed mutagenesis, mutations lead to increased thermal and pH stability, overview | Trichoderma reesei |
S110C/N154C/Q162Y | site-directed mutagenesis, mutations lead to increased thermal and pH stability, overview | Trichoderma reesei |
S110C/N154C/Q162Y/N11D | site-directed mutagenesis, mutations lead to increased thermal and pH stability, overview | Trichoderma reesei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Trichoderma reesei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
x * 21000-22000, native wild-type enzyme, SDS-PAGE, x * 25000, recombinant wild-type enzyme, SDS-PAGE | Trichoderma reesei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichoderma reesei | - |
- |
- |
Subunits | Comment | Organism |
---|---|---|
? | x * 21000-22000, native wild-type enzyme, SDS-PAGE, x * 25000, recombinant wild-type enzyme, SDS-PAGE | Trichoderma reesei |
Synonyms | Comment | Organism |
---|---|---|
endo-1,4-beta-xylanase II | - |
Trichoderma reesei |
More | enzyme belongs to family 11 | Trichoderma reesei |
XYNII | - |
Trichoderma reesei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
assay at | Trichoderma reesei |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
temperature-dependence of activity of wild-type and mutant enzymes | Trichoderma reesei |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
half-lives: wild-type enzyme below 1 min, S110C/N154C 14 min, S110C/N154C/Q162H and S110C/N154C/Q162Y 63 min, S110C/N154C/Q162H/N11D 107 min, S110C/N154C/Q162Y/N11D 97 min, S110C/N154C/Q162H/N11D/N38D 112 min | Trichoderma reesei |
70 | - |
10 min: inactivation of mutant S110C/N154C, 50-60% remaining activity of mutants S110C/N154C/Q162H/N11D, S110C/N154C/Q162Y/N11D, and S110C/N154C/Q162H/N11D/N38D | Trichoderma reesei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 6 | - |
Trichoderma reesei |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pH-dependence of activity of wild-type and mutant enzymes | Trichoderma reesei |