Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Acrophialophora nainiana | |
DTNB | - |
Acrophialophora nainiana | |
DTT | - |
Acrophialophora nainiana | |
L-cysteine | - |
Acrophialophora nainiana | |
L-tryptophan | - |
Acrophialophora nainiana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-bromosuccimide | - |
Acrophialophora nainiana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Acrophialophora nainiana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | secretion | Acrophialophora nainiana | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27500 | - |
2 * 27500, SDS-PAGE | Acrophialophora nainiana |
54000 | - |
gel filtration | Acrophialophora nainiana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acrophialophora nainiana | - |
XynIII | - |
Acrophialophora nainiana XynIII | - |
XynIII | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Acrophialophora nainiana |
Purification (Comment) | Organism |
---|---|
from culture supernatant, 22.12fold to homogeneity by ultrafiltration and a combination of ion exchange and gel filtration chromatography | Acrophialophora nainiana |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
31.25 | - |
purified enzyme | Acrophialophora nainiana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
xylan + H2O | preferred substrate, soluble and insoluble substrate from oat spelt or birchwood | Acrophialophora nainiana | ? | - |
? | |
xylan + H2O | preferred substrate, soluble and insoluble substrate from oat spelt or birchwood | Acrophialophora nainiana XynIII | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 27500, SDS-PAGE | Acrophialophora nainiana |
Synonyms | Comment | Organism |
---|---|---|
beta-xylanase | - |
Acrophialophora nainiana |
xylanase | - |
Acrophialophora nainiana |
XynIII | - |
Acrophialophora nainiana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | 60 | - |
Acrophialophora nainiana |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 65 | rapid decline of activity at 65°C | Acrophialophora nainiana |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
half-life 120 h | Acrophialophora nainiana |
60 | - |
half-life 2 h | Acrophialophora nainiana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
xylan | insoluble oat spelt xylan, pH 6.5, 55°C | Acrophialophora nainiana | |
0.058 | - |
xylan | soluble birchwood xylan, pH 6.5, 55°C | Acrophialophora nainiana | |
0.085 | - |
xylan | insoluble birchwood xylan, pH 6.5, 55°C | Acrophialophora nainiana | |
0.099 | - |
xylan | soluble oat spelt xylan, pH 6.5, 55°C | Acrophialophora nainiana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Acrophialophora nainiana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 8.5 | 50% of maximal activity at pH 4.0 and pH 8.5 | Acrophialophora nainiana |