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Literature summary for 3.2.1.8 extracted from

  • Bakalova, N.G.; Petrova, S.D.; Atev, A.P.; Bhat, M.K.; Kolev, D.N.
    Biochemical and catalytic properties of endo-1,4-beta-xylanases from Thermomyces lanuginosus (wild and mutant strains) (2002), Biotechnol. Lett., 24, 1167-1172.
    View publication on PubMed

Application

Application Comment Organism
paper production use in treatment of paper pulps Thermomyces lanuginosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Thermomyces lanuginosus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Thermomyces lanuginosus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22000
-
wild-type enzyme, gel filtration Thermomyces lanuginosus
24000
-
mutant enzyme, gel filtration Thermomyces lanuginosus

Organism

Organism UniProt Comment Textmining
Thermomyces lanuginosus
-
strain ATCC 44008, cellulase-free wild-type R-MB and mutant M1
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 0.74% carbohydrate content of the wild-type enzyme, 11.8% of the mutant enzyme Thermomyces lanuginosus

Purification (Commentary)

Purification (Comment) Organism
native wild-type, 17fold, and mutant enzymes, to homogeneity by anion exchange chromatography and gel filtration Thermomyces lanuginosus

Reaction

Reaction Comment Organism Reaction ID
(Xylbeta(1-4))n + H2O = (Xylbeta(1-4))n-m + (Xylbeta(1-4))m histidine, tryptophan residues and carboxylic groups are involved in substrate binding or catalysis Thermomyces lanuginosus

Source Tissue

Source Tissue Comment Organism Textmining
additional information organism is grown on corn cobs Thermomyces lanuginosus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1660
-
purified wild-type enzyme Thermomyces lanuginosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-O-methyl-glucuronoxylan + H2O
-
Thermomyces lanuginosus ?
-
?
additional information no activity with mannan, araban, galactomannan, inulin, Avicel, soluble starch, sodium carboxymethylcellulose, 2- or 4-nitrophenyl-gluco-, xylo-, manno-, galacto-pyranosides, and arabinofuranoside Thermomyces lanuginosus ?
-
?
xylan + H2O commercial substrate and from oat spelts and larch sawdust, the latter being the best substrate for wild-type and mutant enzyme Thermomyces lanuginosus ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 22000, wild-type enzyme, SDS-PAGE, 1 * 24000, mutant enzyme, SDS-PAGE Thermomyces lanuginosus

Synonyms

Synonyms Comment Organism
More the enzyme belongs to family 11 of glycosyl hydrolases Thermomyces lanuginosus
xylanase
-
Thermomyces lanuginosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
wild-type enzyme Thermomyces lanuginosus
70
-
mutant enzyme Thermomyces lanuginosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
mutant enzyme retains over 50% activity after 2 h Thermomyces lanuginosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.7
-
mutant enzyme Thermomyces lanuginosus
7
-
wild-type enzyme Thermomyces lanuginosus

pH Range

pH Minimum pH Maximum Comment Organism
3 9
-
Thermomyces lanuginosus

pH Stability

pH Stability pH Stability Maximum Comment Organism
8.3
-
wild-type and mutant enzymes, stable up to 24 h at 50°C, 60% remaining activity Thermomyces lanuginosus

pI Value

Organism Comment pI Value Maximum pI Value
Thermomyces lanuginosus mutant enzyme
-
6
Thermomyces lanuginosus wild-type enzyme
-
6