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Literature summary for 3.2.1.8 extracted from
- Purification, characterization and substrate specificities of xylanase isoenzymes from Melanocarpus albomyces IIS 68 (2000), Biosci. Biotechnol. Biochem., 64, 1173-1180.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| iodoacetic acid | activation of isozymes Ic and IIB | Melanocarpus albomyces |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | inhibition of isozyme Ic | Melanocarpus albomyces | |
| Co2+ | inhibition of isozyme Ic | Melanocarpus albomyces | |
| Fe3+ | inhibition of isozyme Ic | Melanocarpus albomyces | |
| Hg2+ | inhibition of isozymes Ic and IIb | Melanocarpus albomyces | |
| Mn2+ | inhibition of isozyme Ic | Melanocarpus albomyces | |
| N-bromosuccinimide | inhibition of isozymes Ic and IIb | Melanocarpus albomyces | |
| SDS | inhibition of isozyme IIb | Melanocarpus albomyces | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km in mg/ml | Melanocarpus albomyces |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | activation of isozyme IIb | Melanocarpus albomyces | |
| Ca2+ | activation of isozyme IIb | Melanocarpus albomyces | |
| Co2+ | activation of isozyme IIb | Melanocarpus albomyces | |
| Mg2+ | activation of isozymes Ic and IIb | Melanocarpus albomyces | |
| Mn2+ | activation of isozyme IIb | Melanocarpus albomyces | |
| Ni2+ | activation of isozymes Ic and IIb | Melanocarpus albomyces | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 17100 | - |
isozyme Ic, gel filtration | Melanocarpus albomyces |
| 18600 | - |
1 * 22900, isozyme Ia, SDS-PAGE, 1 * 20700, isozyme Ib, SDS-PAGE, 1 * 18600, isozyme Ic, SDS-PAGE, 1 * 31300, isozyme IIa, SDS-PAGE, 1 * 25400, isozyme IIb, SDS-PAGE, 1 * 38500, isozyme IIc, SDS-PAGE, 1 * 34300, isozyme IId, SDS-PAGE | Melanocarpus albomyces |
| 20500 | - |
isozyme Ib, gel filtration | Melanocarpus albomyces |
| 20700 | - |
1 * 22900, isozyme Ia, SDS-PAGE, 1 * 20700, isozyme Ib, SDS-PAGE, 1 * 18600, isozyme Ic, SDS-PAGE, 1 * 31300, isozyme IIa, SDS-PAGE, 1 * 25400, isozyme IIb, SDS-PAGE, 1 * 38500, isozyme IIc, SDS-PAGE, 1 * 34300, isozyme IId, SDS-PAGE | Melanocarpus albomyces |
| 22900 | - |
1 * 22900, isozyme Ia, SDS-PAGE, 1 * 20700, isozyme Ib, SDS-PAGE, 1 * 18600, isozyme Ic, SDS-PAGE, 1 * 31300, isozyme IIa, SDS-PAGE, 1 * 25400, isozyme IIb, SDS-PAGE, 1 * 38500, isozyme IIc, SDS-PAGE, 1 * 34300, isozyme IId, SDS-PAGE | Melanocarpus albomyces |
| 23700 | - |
isozyme Ia, gel filtration | Melanocarpus albomyces |
| 25100 | - |
isozyme IIb, gel filtration | Melanocarpus albomyces |
| 25400 | - |
1 * 22900, isozyme Ia, SDS-PAGE, 1 * 20700, isozyme Ib, SDS-PAGE, 1 * 18600, isozyme Ic, SDS-PAGE, 1 * 31300, isozyme IIa, SDS-PAGE, 1 * 25400, isozyme IIb, SDS-PAGE, 1 * 38500, isozyme IIc, SDS-PAGE, 1 * 34300, isozyme IId, SDS-PAGE | Melanocarpus albomyces |
| 31300 | - |
1 * 22900, isozyme Ia, SDS-PAGE, 1 * 20700, isozyme Ib, SDS-PAGE, 1 * 18600, isozyme Ic, SDS-PAGE, 1 * 31300, isozyme IIa, SDS-PAGE, 1 * 25400, isozyme IIb, SDS-PAGE, 1 * 38500, isozyme IIc, SDS-PAGE, 1 * 34300, isozyme IId, SDS-PAGE | Melanocarpus albomyces |
| 31700 | - |
isozyme IIa, gel filtration | Melanocarpus albomyces |
| 32200 | - |
isozyme IId, gel filtration | Melanocarpus albomyces |
| 34300 | - |
1 * 22900, isozyme Ia, SDS-PAGE, 1 * 20700, isozyme Ib, SDS-PAGE, 1 * 18600, isozyme Ic, SDS-PAGE, 1 * 31300, isozyme IIa, SDS-PAGE, 1 * 25400, isozyme IIb, SDS-PAGE, 1 * 38500, isozyme IIc, SDS-PAGE, 1 * 34300, isozyme IId, SDS-PAGE | Melanocarpus albomyces |
| 38500 | - |
1 * 22900, isozyme Ia, SDS-PAGE, 1 * 20700, isozyme Ib, SDS-PAGE, 1 * 18600, isozyme Ic, SDS-PAGE, 1 * 31300, isozyme IIa, SDS-PAGE, 1 * 25400, isozyme IIb, SDS-PAGE, 1 * 38500, isozyme IIc, SDS-PAGE, 1 * 34300, isozyme IId, SDS-PAGE | Melanocarpus albomyces |
| 39800 | - |
isozyme IIc, gel filtration | Melanocarpus albomyces |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Melanocarpus albomyces | - |
isozymes: Ia, Ib, Ic, IIa, IIb, IIc, IId, only isozyme Ic is constitutive | - |
| Melanocarpus albomyces IIS 68 | - |
isozymes: Ia, Ib, Ic, IIa, IIb, IIc, IId, only isozyme Ic is constitutive | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 7 isozymes to homogeneity by gel filtration and ion-exchange chromatography, overview | Melanocarpus albomyces |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
activity with different xylans, overview | Melanocarpus albomyces |
| 3.33 | - |
isozyme IIa, substrate oat spelt xylan | Melanocarpus albomyces |
| 3.91 | - |
isozyme IId, substrate oat spelt xylan | Melanocarpus albomyces |
| 6.31 | - |
isozyme IIc, substrate oat spelt xylan | Melanocarpus albomyces |
| 11.68 | - |
isozyme IIb, substrate oat spelt xylan | Melanocarpus albomyces |
| 36.58 | - |
isozyme Ib, substrate oat spelt xylan | Melanocarpus albomyces |
| 48.74 | - |
isozyme Ia, substrate oat spelt xylan | Melanocarpus albomyces |
| 142.7 | - |
isozyme Ic, substrate oat spelt xylan | Melanocarpus albomyces |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-xylopyranoside + H2O | only isozyme IIa shows beta-xylosidase activity | Melanocarpus albomyces | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
| 4-nitrophenyl beta-D-xylopyranoside + H2O | only isozyme IIa shows beta-xylosidase activity | Melanocarpus albomyces IIS 68 | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
| beechwood xylan + H2O | - |
Melanocarpus albomyces | ? | - |
? | |
| beechwood xylan + H2O | - |
Melanocarpus albomyces IIS 68 | ? | - |
? | |
| birchwood xylan + H2O | - |
Melanocarpus albomyces | ? | - |
? | |
| birchwood xylan + H2O | - |
Melanocarpus albomyces IIS 68 | ? | - |
? | |
| additional information | substrate specificity for different xylans, overview, isozyme Ia, Ib, Ic, and IIa show also arabinose-releasing property on larchwood xylan | Melanocarpus albomyces | ? | - |
? | |
| additional information | substrate specificity for different xylans, overview, isozyme Ia, Ib, Ic, and IIa show also arabinose-releasing property on larchwood xylan | Melanocarpus albomyces IIS 68 | ? | - |
? | |
| xylan + H2O | substrate specificity of isozymes with different xylans: isozymes Ia, Ib, and Ic prefer larchwood arabinoglucurono xylan, isozymes IIa and IIc prefer birchwood glucurono xylan, isozyme IIb and IId prefer beechwood glucuronoxylan, another substrate is xylan from oat spelt, solka floc xylan is utilized only by isozyme Ic | Melanocarpus albomyces | xylooligosaccharides + D-xylose + xylobiose | isozyme Ia produces only xylooligosaccharides | ? | |
| xylan + H2O | substrate specificity of isozymes with different xylans: isozymes Ia, Ib, and Ic prefer larchwood arabinoglucurono xylan, isozymes IIa and IIc prefer birchwood glucurono xylan, isozyme IIb and IId prefer beechwood glucuronoxylan, another substrate is xylan from oat spelt, solka floc xylan is utilized only by isozyme Ic | Melanocarpus albomyces IIS 68 | xylooligosaccharides + D-xylose + xylobiose | isozyme Ia produces only xylooligosaccharides | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 22900, isozyme Ia, SDS-PAGE, 1 * 20700, isozyme Ib, SDS-PAGE, 1 * 18600, isozyme Ic, SDS-PAGE, 1 * 31300, isozyme IIa, SDS-PAGE, 1 * 25400, isozyme IIb, SDS-PAGE, 1 * 38500, isozyme IIc, SDS-PAGE, 1 * 34300, isozyme IId, SDS-PAGE | Melanocarpus albomyces |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| xylanase | - |
Melanocarpus albomyces |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
isozyme IIc | Melanocarpus albomyces |
| 60 | - |
isozymes IIa and IIb | Melanocarpus albomyces |
| 65 | - |
isozyme IId | Melanocarpus albomyces |
| 70 | - |
isozymes Ia, Ib, and Ic | Melanocarpus albomyces |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
half-lives of the 7 isozymes at different temperatures, isozyme Ia is the most stable, overview | Melanocarpus albomyces |
| 60 | - |
isozyme IIb shows a half-life of 94.5 min, isozyme IId of 85.6 min, isozyme Ia of 164.3 min | Melanocarpus albomyces |
| 70 | - |
isozyme Ia shows a half-life of 16.7 min, isozyme IId of 15.6 min | Melanocarpus albomyces |
| 80 | - |
all isozymes are inactivated within 10 min | Melanocarpus albomyces |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 10.5 | - |
birchwood xylan | isozyme IIc, pH 6.0, 70°C | Melanocarpus albomyces | |
| 10.7 | - |
beechwood xylan | isozyme IId, pH 6.0, 70°C | Melanocarpus albomyces | |
| 13.9 | - |
larchwood xylan | isozyme Ia, pH 6.0, 70°C | Melanocarpus albomyces | |
| 41.2 | - |
larchwood xylan | isozyme Ib, pH 6.0, 70°C | Melanocarpus albomyces | |
| 53.4 | - |
larchwood xylan | isozyme Ic, pH 6.0, 70°C | Melanocarpus albomyces | |
| 73 | - |
birchwood xylan | isozyme IIa, pH 6.0, 70°C | Melanocarpus albomyces | |
| 83.8 | - |
beechwood xylan | isozyme IIb, pH 6.0, 70°C | Melanocarpus albomyces |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
isozyme Ib | Melanocarpus albomyces |
| 6 | - |
isozymes Ia and Ic | Melanocarpus albomyces |
| 7 | - |
isozymes IIb and IId | Melanocarpus albomyces |
| 8 | - |
isozyme IIc | Melanocarpus albomyces |
| 8.5 | - |
isozyme IIa | Melanocarpus albomyces |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Melanocarpus albomyces | isozyme IIa, isoelectric focusing | - |
4.2 |
| Melanocarpus albomyces | isozyme IIb, isoelectric focusing | - |
4.2 |
| Melanocarpus albomyces | isozyme Ia, isoelectric focusing | - |
6 |
| Melanocarpus albomyces | isozyme Ib, isoelectric focusing | - |
6 |
| Melanocarpus albomyces | isozyme Ic, isoelectric focusing | - |
6 |
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