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Literature summary for 3.2.1.8 extracted from
- Purification and properties of a beta-1,4-xylanase from a cellulolytic extreme thermophile expressed in Escherichia coli (1993), Int. J. Biochem., 25, 609-617.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Caldicellulosiruptor saccharolyticus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| iodoacetate | 5 mM, 21% loss of activity | Caldicellulosiruptor saccharolyticus |  |
| Ni2+ | 5 mM, complete inhibition | Caldicellulosiruptor saccharolyticus | |
| RbCl | 5 mM, 55% inhibition | Caldicellulosiruptor saccharolyticus | |
| SDS | 1% w/w, 86% loss of activity | Caldicellulosiruptor saccharolyticus | |
| xylose | no inhibition | Caldicellulosiruptor saccharolyticus | |
| Zn2+ | 5 mM, complete inhibition | Caldicellulosiruptor saccharolyticus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | 200 mM, slight activation | Caldicellulosiruptor saccharolyticus | |
| Li+ | 200 mM, slight activation | Caldicellulosiruptor saccharolyticus | |
| Mg2+ | 5 mM MgCl2, slight activation | Caldicellulosiruptor saccharolyticus | |
| Na+ | 200 mM NaCl, slight activation | Caldicellulosiruptor saccharolyticus | |
| SrCl2 | 5 mM, slight activation | Caldicellulosiruptor saccharolyticus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
gel filtration | Caldicellulosiruptor saccharolyticus |
| 42000 | - |
1 * 42000, SDS-PAGE | Caldicellulosiruptor saccharolyticus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Caldicellulosiruptor saccharolyticus | - |
- |
- |
| Caldicellulosiruptor saccharolyticus Tp8T6.3.3.1 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme expressed in Escherichia coli | Caldicellulosiruptor saccharolyticus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 167 | - |
- |
Caldicellulosiruptor saccharolyticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,4-beta-D-xylan + H2O | low activity on larchwood xylan | Caldicellulosiruptor saccharolyticus | additional information | release of xylobiose and some xylotriose from oat spelt xylan | ? | |
| 1,4-beta-D-xylan + H2O | larchwood xylan | Caldicellulosiruptor saccharolyticus | additional information | release of xylobiose and some xylotriose from oat spelt xylan | ? | |
| 1,4-beta-D-xylan + H2O | oat spelt xylan | Caldicellulosiruptor saccharolyticus | additional information | release of xylobiose and some xylotriose from oat spelt xylan | ? | |
| 1,4-beta-D-xylan + H2O | low activity on larchwood xylan | Caldicellulosiruptor saccharolyticus Tp8T6.3.3.1 | additional information | release of xylobiose and some xylotriose from oat spelt xylan | ? | |
| 1,4-beta-D-xylan + H2O | larchwood xylan | Caldicellulosiruptor saccharolyticus Tp8T6.3.3.1 | additional information | release of xylobiose and some xylotriose from oat spelt xylan | ? | |
| 1,4-beta-D-xylan + H2O | oat spelt xylan | Caldicellulosiruptor saccharolyticus Tp8T6.3.3.1 | additional information | release of xylobiose and some xylotriose from oat spelt xylan | ? | |
| 4-methylumbelliferyl beta-D-cellobioside + H2O | - |
Caldicellulosiruptor saccharolyticus | ? | - |
? | |
| acetylhemicellulose + H2O | high activity on sugar cane bagasse hemicellulose A and B | Caldicellulosiruptor saccharolyticus | hemicellulose + acetate | - |
? | |
| acetylhemicellulose + H2O | high activity on sugar cane bagasse hemicellulose A and B | Caldicellulosiruptor saccharolyticus Tp8T6.3.3.1 | hemicellulose + acetate | - |
? | |
| carboxymethylcellulose + H2O | - |
Caldicellulosiruptor saccharolyticus | ? | - |
? | |
| p-nitrophenyl beta-D-cellobioside + H2O | - |
Caldicellulosiruptor saccharolyticus | ? | - |
? | |
| p-nitrophenyl beta-D-xylopyranoside | transferase activity on | Caldicellulosiruptor saccharolyticus | ? | - |
? | |
| xylobiose + H2O | no hydrolysis | Caldicellulosiruptor saccharolyticus | ? | - |
? | |
| xylobiose + H2O | no hydrolysis | Caldicellulosiruptor saccharolyticus Tp8T6.3.3.1 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 42000, SDS-PAGE | Caldicellulosiruptor saccharolyticus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Caldicellulosiruptor saccharolyticus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 80 | 40°C: about 30% of maximal activity, 80°C: about 20% of maximal activity | Caldicellulosiruptor saccharolyticus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
pH 6.0, half-life: 4 min in absence of added protein, 30 min, in presence of 0.5 mg/ml bovine serum albumin | Caldicellulosiruptor saccharolyticus |
| 80 | - |
pH 6.0, half-life is 20 min in presence of 0.5 mg/ml bovine serum albumin | Caldicellulosiruptor saccharolyticus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Caldicellulosiruptor saccharolyticus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7.7 | more than 50% of maximal activity at pH 5.0 and at pH 7.0 | Caldicellulosiruptor saccharolyticus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 11.5 | stable at room temperature in presence of 0.5 mg/ml bovine serum albumin | Caldicellulosiruptor saccharolyticus |
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