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Literature summary for 3.2.1.8 extracted from

  • Bataillon, M.; Cardinali, A.P.N.; Castillon, N.; Duchiron, F.
    Purification and characterization of a moderately thermostable xylanase from Bacillus sp. strain SPS-0 (2000), Enzyme Microb. Technol., 26, 187-192.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
beta-mercaptoethanol 10 mM, stimulates Bacillus sp. (in: Bacteria)
dithiothreitol stimulates Bacillus sp. (in: Bacteria)

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis-(2-nitrobenzoic acid)
-
Bacillus sp. (in: Bacteria)
Cu2+ 1 mM, 30% loss of activity Bacillus sp. (in: Bacteria)
DTNB
-
Bacillus sp. (in: Bacteria)
Hg2+ 1 mM, complete inhibition Bacillus sp. (in: Bacteria)
N-bromosuccinimide
-
Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Bacillus sp. (in: Bacteria)

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ stimulates Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
99000
-
x * 99000, SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
-
-
Bacillus sp. (in: Bacteria) SPS-0
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,4-beta-D-xylan + H2O birchwood xylan Bacillus sp. (in: Bacteria) additional information xylose + xylobiose + xylotriose + xylotetraose ?
1,4-beta-D-xylan + H2O birchwood xylan Bacillus sp. (in: Bacteria) SPS-0 additional information xylose + xylobiose + xylotriose + xylotetraose ?
arabinoxylan + H2O from wheat bran Bacillus sp. (in: Bacteria) additional information
-
?
arabinoxylan + H2O from wheat bran Bacillus sp. (in: Bacteria) SPS-0 additional information
-
?

Subunits

Subunits Comment Organism
? x * 99000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
-
Bacillus sp. (in: Bacteria)

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60 80 60°C: about 50% of maximal activity, 80°C: about 80% of maximal activity Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
stable Bacillus sp. (in: Bacteria)
70
-
pH 6.0, 4 h, 20% loss of activity in presence of xylan, 80% loss of activity without xylan Bacillus sp. (in: Bacteria)
70
-
4 h, in presence of xylan, 20% loss of activity Bacillus sp. (in: Bacteria)
80
-
15 min, complete loss of activity Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7
-
Bacillus sp. (in: Bacteria)
6
-
-
Bacillus sp. (in: Bacteria)

pH Range

pH Minimum pH Maximum Comment Organism
5 9 pH 5.0: about 50% of maximal activity, pH 9.0: about 60% of maximal activity Bacillus sp. (in: Bacteria)