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Literature summary for 3.2.1.8 extracted from

  • Gupta, S.; Bhushnan, B.; Hoondal, G.S.
    Isolation, purification and characterization of xylanase from Staphylococcus sp. SG-13 and its application in biobleaching of kraft pulp (2000), J. Appl. Microbiol., 88, 325-334.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol stimulates Staphylococcus sp.

Application

Application Comment Organism
paper production biobleaching of eucalyptus kraft pulp Staphylococcus sp.

Inhibitors

Inhibitors Comment Organism Structure
Acetic anhydride
-
Staphylococcus sp.
Co2+
-
Staphylococcus sp.
EDTA
-
Staphylococcus sp.
Hg2+
-
Staphylococcus sp.
phenyl methane sulfonyl fluoride
-
Staphylococcus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Staphylococcus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ stimulates Staphylococcus sp.
Fe2+ stimulates Staphylococcus sp.
Ni2+ stimulates Staphylococcus sp.

Organism

Organism UniProt Comment Textmining
Staphylococcus sp.
-
-
-
Staphylococcus sp. SG-13
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Staphylococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,4-beta-D-xylan + H2O birchwood xylan Staphylococcus sp. additional information
-
?
1,4-beta-D-xylan + H2O oat spelt xylan Staphylococcus sp. additional information
-
?
1,4-beta-D-xylan + H2O birchwood xylan Staphylococcus sp. SG-13 additional information
-
?
1,4-beta-D-xylan + H2O oat spelt xylan Staphylococcus sp. SG-13 additional information
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
half-life: 240 min Staphylococcus sp.
55
-
half-life: 30 min Staphylococcus sp.
60
-
half-life 10 min Staphylococcus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
and a second optimum at pH 9.2 Staphylococcus sp.
9.2
-
and a second optimum at pH 7.5 Staphylococcus sp.

pH Range

pH Minimum pH Maximum Comment Organism
7.5 9.2 more than 70% of maximal activity at pH 6.0 and at 10.5 Staphylococcus sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.5 9.5 50°C, 1 h, retains more than 90% of its activity Staphylococcus sp.