Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 1 mM | Malbranchea pulchella | |
Hg2+ | 0.1 mM | Malbranchea pulchella |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21000 | - |
x * 21000, xylanase A, SDS-PAGE | Malbranchea pulchella |
30000 | - |
x * 30000, xylanase B, SDS-PAGE | Malbranchea pulchella |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Malbranchea pulchella | - |
var. sulfurea | - |
Purification (Comment) | Organism |
---|---|
- |
Malbranchea pulchella |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Malbranchea pulchella |
1475 | - |
xylanase A | Malbranchea pulchella |
1582 | - |
xylanase B | Malbranchea pulchella |
Storage Stability | Organism |
---|---|
4°C, 30 days, stable | Malbranchea pulchella |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,4-beta-D-xylan + H2O | - |
Malbranchea pulchella | additional information | - |
? | |
arabinoxylan + H2O | - |
Malbranchea pulchella | additional information | xylobiose + arabinotetraose + smaller amounts of xylose, arabinoxylopentaose, xylotriose and traces of branched arabino-xylose oligosaccharides with a degree of polymerization from 4-9. Xylanase B produces less xylose but more xylotriose and xylotetraose than xylanase A | ? | |
additional information | xylanase B shows high glycosyltransferase activity | Malbranchea pulchella | ? | - |
? | |
xylobiose + H2O | no hydrolysis | Malbranchea pulchella | ? | - |
? | |
xyloheptaose + H2O | - |
Malbranchea pulchella | xylobiose + xylotriose + xylotetraose | - |
? | |
xylooctaose + H2O | - |
Malbranchea pulchella | xylobiose + xylotriose + xylotetraose | - |
? | |
xylopentaose + H2O | - |
Malbranchea pulchella | additional information | xylose + xylobiose + xylotriose | ? | |
xylotetraose + H2O | - |
Malbranchea pulchella | additional information | - |
? | |
xylotriose + H2O | weak activity | Malbranchea pulchella | xylobiose + xylose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 30000, xylanase B, SDS-PAGE | Malbranchea pulchella |
? | x * 21000, xylanase A, SDS-PAGE | Malbranchea pulchella |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
xylanase A and B | Malbranchea pulchella |
70 | - |
- |
Malbranchea pulchella |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
1 h, xylanase A is stable, 25% loss of activity of xylanase B | Malbranchea pulchella |
50 | - |
pH 6.7, 30 min, stable | Malbranchea pulchella |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.5 | - |
xylanase A | Malbranchea pulchella |
6 | 6.5 | - |
Malbranchea pulchella |
6.5 | - |
xylanase B | Malbranchea pulchella |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2.5 | 6 | xylanase A, stable | Malbranchea pulchella |
3.5 | 8 | xylanase B, stable | Malbranchea pulchella |
6.7 | - |
50°C, 30 min, stable | Malbranchea pulchella |