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Literature summary for 3.2.1.8 extracted from

  • Sakka, K.; Kojima, Y.; Kondo, T.; Karita, S.; Shimada, K.; Ohmiya, K.
    Purification and characterization of xylanase A from Clostridium stercorarium F-9 and a recombinant Escherichia coli (1994), Biosci. Biotechnol. Biochem., 58, 1496-1499.
    View publication on PubMed
Search for more literature for 3.2.1.8

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 1 mM CuCl2, patial inhibition, xylanase A Thermoclostridium stercorarium
Fe2+ 1 mM FeSO4, complete inhibition, xylanase A Thermoclostridium stercorarium
Fe3+ Fe2(SO4)3, 1 mM, complete inhibition, xylanase A Thermoclostridium stercorarium
Hg2+ 1 mM HgCl2, complete inhibition, xylanase A Thermoclostridium stercorarium
PCMB 1 mM, complete inhibition, xylanase A Thermoclostridium stercorarium
Zn2+ 1 mM ZnCl2, patial inhibition, xylanase A Thermoclostridium stercorarium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Thermoclostridium stercorarium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
 x * 54000, recombinant xylanase A produced in Escherichia coli, SDS-PAGE Thermoclostridium stercorarium

Organism

Organism UniProt Comment Textmining
Thermoclostridium stercorarium
-
 xylanase A
-
Thermoclostridium stercorarium F-9
-
 xylanase A
-

Purification (Commentary)

Purification (Comment) Organism
recombinant xylanase A produced in Escherichia coli Thermoclostridium stercorarium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,4-beta-D-xylan + H2O xylan from oat spelt, xylanase A Thermoclostridium stercorarium additional information
-
 ?
1,4-beta-D-xylan + H2O xylan from oat spelt, xylanase A Thermoclostridium stercorarium F-9 additional information
-
 ?
xylobiose + H2O low activity, xylanase A Thermoclostridium stercorarium ?
-
 ?
xylobiose + H2O low activity, xylanase A Thermoclostridium stercorarium F-9 ?
-
 ?
xylotetraose + H2O xylanase A Thermoclostridium stercorarium additional information xylobiose + small amounts of xylose and xylotriose, xylanase A ?
xylotetraose + H2O xylanase A Thermoclostridium stercorarium F-9 additional information xylobiose + small amounts of xylose and xylotriose, xylanase A ?
xylotriose + H2O xylanase A Thermoclostridium stercorarium xylobiose + xylose xylanase A ?
xylotriose + H2O xylanase A Thermoclostridium stercorarium F-9 xylobiose + xylose xylanase A ?

Subunits

Subunits Comment Organism
? x * 54000, recombinant xylanase A produced in Escherichia coli, SDS-PAGE Thermoclostridium stercorarium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
 xylanase A Thermoclostridium stercorarium

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 10 min, xylanase A, stable Thermoclostridium stercorarium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 xylanase A Thermoclostridium stercorarium