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Literature summary for 3.2.1.68 extracted from

  • Yun, M.S.; Umemoto, T.; Kawagoe, Y.
    Rice debranching enzyme isoamylase3 facilitates starch metabolism and affects plastid morphogenesis (2011), Plant Cell Physiol., 52, 1068-1082.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.68

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Oryza sativa

Localization

Localization Comment Organism GeneOntology No. Textmining
plastid stroma
-
 Oryza sativa 9532
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
79000
-
 x * 79000, SDS-PAGE Oryza sativa

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
 cultivar Nipponbare
-

Source Tissue

Source Tissue Comment Organism Textmining
endosperm
-
 Oryza sativa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin + H2O
-
 Oryza sativa ?
-
 ?
beta-limit dextrin + H2O
-
 Oryza sativa ?
-
 ?
additional information recombinant isoamylase 3 does not readily hydrolyze red pullulan and amylose Oryza sativa ?
-
 ?
starch + H2O
-
 Oryza sativa ?
-
 ?

Subunits

Subunits Comment Organism
? x * 79000, SDS-PAGE Oryza sativa

Synonyms

Synonyms Comment Organism
ISA3
-
 Oryza sativa

General Information

General Information Comment Organism
malfunction both overexpression and loss of function of isoamylase 3 in the endosperm generated pleomorphic amyloplasts and starch granules Oryza sativa
physiological function isoamylase 3 facilitates starch metabolism and affects morphological characteristics of plastids in rice. Both overexpression and loss of function of isoamylase 3 in the endosperm generates pleomorphic amyloplasts and starch granules Oryza sativa