Cloned (Comment) | Organism |
---|---|
expression of OsISA2 in Escherichia coli | Oryza sativa |
expression of OsISA2in Escherichia coli | Oryza sativa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00002 | - |
amylose-extender amylopectin | hetero-oligomer | Oryza sativa | |
0.00004 | - |
amylose-extender amylopectin | homo-oligomer | Oryza sativa | |
0.00012 | - |
amylopectin | hetero-oligomer | Oryza sativa | |
0.00018 | - |
beta-limit dextrin | hetero-oligomer | Oryza sativa | |
0.00021 | - |
beta-limit dextrin | homo-oligomer | Oryza sativa | |
0.00025 | - |
amylopectin | homo-oligomer | Oryza sativa | |
0.00062 | - |
oyster glycogen | hetero-oligomer | Oryza sativa | |
0.00067 | - |
oyster glycogen | homo-oligomer | Oryza sativa | |
0.0054 | - |
phytoglycogen | hetero-oligomer | Oryza sativa | |
0.0065 | - |
phytoglycogen | homo-oligomer | Oryza sativa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
85000 | - |
homooligomer OsISA1 5 * 830000 and heterooligomer OsISA1-OsISA2 6 * 85000, HPLC gel filtration | Oryza sativa |
420000 | - |
SDS-PAGE, homooligomer, five OsISA1 | Oryza sativa |
510000 | - |
SDS-PAGE, heterooligomer, hetero-hexamer composed of five OsISA1 and one OsISA2 | Oryza sativa |
830000 | - |
homooligomer OsISA1 5 * 830000 and heterooligomer OsISA1-OsISA2 6 * 85000, HPLC gel filtration | Oryza sativa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | - |
L. cv. Nipponbare | - |
Oryza sativa | O80403 | L. cv. Nipponbare | - |
Purification (Comment) | Organism |
---|---|
wild type enzyme by HitrapQ HP and TSKgel G3000SWXL column chromatography, recombinant OsISA2 by HitrapQ HP column chromatography | Oryza sativa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
endosperm | - |
Oryza sativa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + H2O | - |
Oryza sativa | maltose + maltooligosaccharides | - |
? | |
amylose-extender amylopectin + H2O | - |
Oryza sativa | maltose + maltooligosaccharides | - |
? | |
beta limit dextrin + H2O | from maize | Oryza sativa | maltose + maltooligosaccharides | - |
? | |
oyster glycogen + H2O | - |
Oryza sativa | ? | - |
? | |
oyster gylcogen + H2O | - |
Oryza sativa | maltose + maltooligosaccharides | - |
? | |
phytoglycogen + H2O | - |
Oryza sativa | maltose + maltooligosaccharides | - |
? | |
starch + H2O | - |
Oryza sativa | maltose + maltooligosaccharides | - |
? |
Subunits | Comment | Organism |
---|---|---|
oligomer | heterooligomer OsISA1-OsISA2 6*85000, HPLC gel filtration | Oryza sativa |
oligomer | homooligomer OsISA1 5 * 830000 and heterooligomer OsISA1-OsISA2 6 * 85000, HPLC gel filtration | Oryza sativa |
Synonyms | Comment | Organism |
---|---|---|
glycogen alpha-1,6-glucanohydrolase | - |
Oryza sativa |
isoamylase | - |
Oryza sativa |
OsISA1 | - |
Oryza sativa |
OsISA2 | - |
Oryza sativa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
homo-oligomer | Oryza sativa |
40 | - |
hetero-oligomer | Oryza sativa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
homooligomer, complete loss of activity | Oryza sativa |
40 | 50 | hetero-oligomer, loss of 50% activity | Oryza sativa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | - |
Oryza sativa |