Cloned (Comment) | Organism |
---|---|
expression of the gene lacking the 30-residue N-terminal secretion signal sequence in Escherichia coli, selenomethionine-derivatized enzyme | Rhodococcus sp. |
Crystallization (Comment) | Organism |
---|---|
by vapor diffusion in the presence of detergents like Triton X-100, octyl beta-D-glucopyranoside, decyl beta-D-maltopyranoside, and dodecyl beta-D-maltopyranoside, form, in complex with the ganglioside GM3 | Rhodococcus sp. |
Protein Variants | Comment | Organism |
---|---|---|
E233A | site directed mutagenesis | Rhodococcus sp. |
E351S | site directed mutagenesis | Rhodococcus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
D-erythro-sphingosine | - |
Rhodococcus sp. | |
0.3 | - |
D-erythro-dihydrosphingosine | - |
Rhodococcus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
of the recombinant protein by by Ni(II) affinity chromatography | Rhodococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-erythro-dihydrosphingosine + a sugar | - |
Rhodococcus sp. | ? | - |
? | |
D-erythro-sphingosine + a sugar | - |
Rhodococcus sp. | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
EGC | - |
Rhodococcus sp. |
endo-glycoceramidase II | membrane-associated family 5 glycosidase | Rhodococcus sp. |
glycosylceramidase | - |
Rhodococcus sp. |