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Literature summary for 3.2.1.62 extracted from

  • Beau, I.; Cotte-Laffitte, J.; Geniteau-Legendre, M.; Estes, M.K.; Servin, A.L.
    An NSP4-dependant mechanism by which rotavirus impairs lactase enzymatic activity in brush border of human enterocyte-like Caco-2 cells (2007), Cell. Microbiol., 9, 2254-2266.
    View publication on PubMed
Search for more literature for 3.2.1.62

Application

Application Comment Organism
medicine rotavirus infection impairs LPH activity in intestinal cells, the decrease in enzyme activity is not Ca2+- and cAMP-dependent, the LPH biosynthesis, stability, and expression is not modified, the impairment of lactase enzymatic activity during rotavirus infection results from an inhibitory action of the secreted non-structural rotavirus protein NSP4 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
NSP4 secreted non-structural rotavirus protein Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
28.9
-
 lactose in infected cells, in brush border-membrane enriched fraction Homo sapiens
30
-
 lactose in non infected cells, in brush border-membrane enriched fraction Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
160000
-
 SDS-PAGE, cleaved mature form Homo sapiens
220000
-
 SDS-PAGE, high mannose form Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by magnesium precipitation method Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
Caco-2 cell
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00164
-
 in non infected cells, cell homogenates Homo sapiens
0.00167
-
 in cells infected with rhesus monkey rotavirus, cell homogenates Homo sapiens
0.00219
-
 in cells infected with rhesus monkey rotavirus, in brush border-membrane enriched fraction Homo sapiens
0.0028
-
 in cells infected with rhesus monkey rotavirus and NSP4 neutralizing antibodies, in brush border-membrane enriched fraction Homo sapiens
0.00334
-
 in non infected cells, in brush border-membrane enriched fraction Homo sapiens
0.00359
-
 in cells infected with inactivated rhesus monkey rotavirus, in brush border-membrane enriched fraction Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycosyl-N-acylsphingosine + H2O
-
 Homo sapiens N-acylsphingosine + a sugar
-
 ?
lactose + H2O
-
 Homo sapiens D-glucose + D-galactose
-
 ?

Synonyms

Synonyms Comment Organism
glycosyl-N-acylsphingosine glycohydrolase
-
 Homo sapiens
glycosylceramidase
-
 Homo sapiens
lactase-phlorizin hydrolase brush border membrane (BBM)-associated enzyme in intestinal cells Homo sapiens
LPH
-
 Homo sapiens