Literature summary for 3.2.1.62 extracted from

Wacker, H.; Keller, P.; Falchetto, R.; Legler, G.; Semenza, G.
Location of the two catalytic sites in intestinal lactase-phlorizin hydrolase (1992), J. Biol. Chem., 267, 18744-18752.

Search for more literature for 3.2.1.62

Inhibitors

Inhibitors	Comment	Organism	Structure
conduritol-B-epoxide	90% inactivation of lactase hydrolysis, 55% inactivation of phlorizin hydrolysis, inactivation by covalent binding to Glu1747 and Glu 1271	Oryctolagus cuniculus
deoxygalactonojirimycin	competitive inhibition, Ki: 0.076 mM	Oryctolagus cuniculus
deoxynojirimycin	competitive inhibition, Ki: 0.142 mM	Oryctolagus cuniculus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
brush border	-	Oryctolagus cuniculus	5903	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
135000	-	SDS-PAGE	Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O	Oryctolagus cuniculus	hydrolysis located on a specific active site for lactose hydrolysis	D-galactose + beta-D-glucose	-	?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O	Oryctolagus cuniculus	two enzymatic activities, one for lactose- and one for phlorizin-and lactose-hydrolysis	D-galactose + beta-D-glucose	-	?
phlorizin + H2O	Oryctolagus cuniculus	hydrolysis located on a specific active site for phlorizin hydrolysis	phloretin + beta-D-glucose	-	?

Organism

Organism	UniProt	Comment	Textmining
Oryctolagus cuniculus	-	rabbit	-

Purification (Commentary)

Purification (Comment)	Organism
to homogeneity, chromatography techniques	Oryctolagus cuniculus

Source Tissue

Source Tissue	Comment	Organism	Textmining
intestine	small intestine	Oryctolagus cuniculus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
4.8	-	-	Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O	hydrolysis located on a specific active site for lactose hydrolysis	Oryctolagus cuniculus	D-galactose + beta-D-glucose	-	?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O	two enzymatic activites, one for lactose- and one for phlorizin-and lactose-hydrolysis	Oryctolagus cuniculus	D-galactose + beta-D-glucose	-	?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O	two enzymatic activities, one for lactose- and one for phlorizin-and lactose-hydrolysis	Oryctolagus cuniculus	D-galactose + beta-D-glucose	-	?
phlorizin + H2O	hydrolysis located on a specific active site for phlorizin hydrolysis	Oryctolagus cuniculus	phloretin + beta-D-glucose	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 135000, SDS-PAGE	Oryctolagus cuniculus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.076	-	deoxygalactonojirimycin	competitive inhibition	Oryctolagus cuniculus
0.142	-	deoxynojirimycin	competitive inhibition	Oryctolagus cuniculus

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Release 2023.1 (January 2023)