Literature summary for 3.2.1.52 extracted from

Ryslava, H.; Kalendova, A.; Doubnerova, V.; Skocdopol, P.; Kumar, V.; Kukacka, Z.; Pompach, P.; Vanek, O.; Slamova, K.; Bojarova, P.; Kulik, N.; Ettrich, R.; Kren, V.; Bezouska, K.
Enzymatic characterization and molecular modeling of an evolutionarily interesting fungal beta-N-acetylhexosaminidase (2011), FEBS J., 278, 2469-2484.

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Inhibitors

Inhibitors	Comment	Organism	Structure
4-nitrophenyl 2-acetamido-2-deoxy-beta-D-galactopyranoside	substrate inhibition, competitive	Penicillium oxalicum
4-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranoside	substrate inhibition, noncompetitive	Penicillium oxalicum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.05	-	4-methylumbelliferyl N-acetyl-beta-D-glucosamine	pH 3.0, 20°C	Penicillium oxalicum
0.13	-	4-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranoside	pH 3.0, 20°C	Penicillium oxalicum
1.04	-	4-nitrophenyl 2-acetamido-2-deoxy-beta-D-galactopyranoside	pH 3.0, 20°C	Penicillium oxalicum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
15000	-	2 * 65000, catalytic unit, plus 2 * 15000, propeptide, SDS-PAGE	Penicillium oxalicum
65000	-	2 * 65000, catalytic unit, plus 2 * 15000, propeptide, SDS-PAGE	Penicillium oxalicum
160000	-	gel filtration	Penicillium oxalicum

Organism

Organism	UniProt	Comment	Textmining
Penicillium oxalicum	-	-	-
Penicillium oxalicum CCF 3438	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
culture fluid	-	Penicillium oxalicum	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-methylumbelliferyl N-acetyl-beta-D-glucosamine + H2O	-	Penicillium oxalicum	4-methylumbelliferone + N-acetylglucosamine	-	?
4-methylumbelliferyl N-acetyl-beta-D-glucosamine + H2O	-	Penicillium oxalicum CCF 3438	4-methylumbelliferone + N-acetylglucosamine	-	?
4-nitrophenyl 2-acetamido-2-deoxy-beta-D-galactopyranoside + H2O	-	Penicillium oxalicum	2-acetamido-2-deoxy-beta-D-galactopyranose + 4-nitrophenol	-	?
4-nitrophenyl 2-acetamido-2-deoxy-beta-D-galactopyranoside + H2O	-	Penicillium oxalicum CCF 3438	2-acetamido-2-deoxy-beta-D-galactopyranose + 4-nitrophenol	-	?
4-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O	-	Penicillium oxalicum	2-acetamido-2-deoxy-beta-D-glucopyranose + 4-nitrophenol	-	?
4-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O	-	Penicillium oxalicum CCF 3438	2-acetamido-2-deoxy-beta-D-glucopyranose + 4-nitrophenol	-	?
4-nitrophenyl 2-formamido-2-deoxy-beta-D-glucopyranoside + H2O	about 12-14% of the activity with 4-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranoside	Penicillium oxalicum	2-formamido-2-deoxy-beta-D-glucopyranose + 4-nitrophenol	-	?
4-nitrophenyl 2-formamido-2-deoxy-beta-D-glucopyranoside + H2O	about 12-14% of the activity with 4-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranoside	Penicillium oxalicum CCF 3438	2-formamido-2-deoxy-beta-D-glucopyranose + 4-nitrophenol	-	?
4-nitrophenyl 2-glycolylamido-2-deoxy-beta-D-glucopyranoside + H2O	about 12-14% of the activity with 4-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranoside	Penicillium oxalicum	2-glycolylamido-2-deoxy-beta-D-glucopyranose + 4-nitrophenol	-	?
4-nitrophenyl 2-propionamido-2-deoxy-beta-D-glucopyranoside + H2O	about 12-14% of the activity with 4-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranoside	Penicillium oxalicum	2-propionamido-2-deoxy-beta-D-glucopyranose + 4-nitrophenol	-	?
additional information	poor substrate: 4-nitrophenyl 2-trifluoroacetamido-2-deoxy-beta-D-glucopyranoside	Penicillium oxalicum	?	-	?
additional information	poor substrate: 4-nitrophenyl 2-trifluoroacetamido-2-deoxy-beta-D-glucopyranoside	Penicillium oxalicum CCF 3438	?	-	?

Subunits

Subunits	Comment	Organism
tetramer	2 * 65000, catalytic unit, plus 2 * 15000, propeptide, SDS-PAGE	Penicillium oxalicum

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	-	Penicillium oxalicum

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
50	-	rapid decrease in activity above	Penicillium oxalicum

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
43	-	4-methylumbelliferyl N-acetyl-beta-D-glucosamine	pH 3.0, 20°C	Penicillium oxalicum
347	-	4-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranoside	pH 3.0, 20°C	Penicillium oxalicum
723	-	4-nitrophenyl 2-acetamido-2-deoxy-beta-D-galactopyranoside	pH 3.0, 20°C	Penicillium oxalicum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
3	-	-	Penicillium oxalicum

pH Range

pH Minimum	pH Maximum	Comment	Organism
2	4	-	Penicillium oxalicum

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
4	-	4-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranoside	pH 3.0, 20°C	Penicillium oxalicum
21	-	4-nitrophenyl 2-acetamido-2-deoxy-beta-D-galactopyranoside	pH 3.0, 20°C	Penicillium oxalicum

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
695	-	4-nitrophenyl 2-acetamido-2-deoxy-beta-D-galactopyranoside	pH 3.0, 20°C	Penicillium oxalicum
860	-	4-methylumbelliferyl N-acetyl-beta-D-glucosamine	pH 3.0, 20°C	Penicillium oxalicum
2669	-	4-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranoside	pH 3.0, 20°C	Penicillium oxalicum

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Release 2023.1 (January 2023)