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Literature summary for 3.2.1.52 extracted from
- Gene cloning, expression, and characterization of a second beta-N-acetylglucosaminidase from the chitinolytic bacterium Aeromonas hydrophila strain SUWA-9 (2008), Biosci. Biotechnol. Biochem., 72, 492-498.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | nagB is transcribed when SUWA-9 cells are grown in the presence of colloidal chitin, but not glucose | Aeromonas hydrophila |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| ligated with vector pQE-30 and expressed in Escherichia coli BL21(DE3) | Aeromonas hydrophila |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 8.57 | - |
4-nitrophenyl-N-acetyl-beta-D-glucosaminide | - |
Aeromonas hydrophila |  |
| 11.1 | - |
4-nitrophenyl beta-N-acetyl-D-galactosaminide | - |
Aeromonas hydrophila | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 69000 | - |
x * 69000, SDS-PAGE | Aeromonas hydrophila |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aeromonas hydrophila | - |
- |
- |
| Aeromonas hydrophila SUWA-9 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by ion exchange and gel filtration, to homogeneity, 3.9fold | Aeromonas hydrophila |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl N-acetyl-beta-D-galactosaminide + H2O | - |
Aeromonas hydrophila | 4-nitrophenol + N-acetyl-beta-D-galactosamine | - |
? | |
| 4-nitrophenyl N-acetyl-beta-D-galactosaminide + H2O | - |
Aeromonas hydrophila SUWA-9 | 4-nitrophenol + N-acetyl-beta-D-galactosamine | - |
? | |
| 4-nitrophenyl-diacetylchitobiose + H2O | - |
Aeromonas hydrophila | 4-nitrophenol + N-acetyl-beta-D-glucosamine | - |
? | |
| 4-nitrophenyl-diacetylchitobiose + H2O | - |
Aeromonas hydrophila SUWA-9 | 4-nitrophenol + N-acetyl-beta-D-glucosamine | - |
? | |
| 4-nitrophenyl-N-acetyl-beta-D-glucosaminide + H2O | - |
Aeromonas hydrophila | 4-nitrophenol + N-acetyl-beta-D-glucosamine | - |
? | |
| 4-nitrophenyl-N-acetyl-beta-D-glucosaminide + H2O | - |
Aeromonas hydrophila SUWA-9 | 4-nitrophenol + N-acetyl-beta-D-glucosamine | - |
? | |
| 4-nitrophenyl-N-acetylchitotetraose + H2O | - |
Aeromonas hydrophila | 4-nitrophenol + N-acetyl-beta-D-glucosamine | - |
? | |
| 4-nitrophenyl-N-acetylchitotetraose + H2O | - |
Aeromonas hydrophila SUWA-9 | 4-nitrophenol + N-acetyl-beta-D-glucosamine | - |
? | |
| 4-nitrophenyl-N-acetylchitotriose + H2O | - |
Aeromonas hydrophila | 4-nitrophenol + N-acetyl-beta-D-glucosamine | - |
? | |
| additional information | does not hydrolyze 4-nitrophenyl-beta-glucoside nor 4-nitrophenyl-beta-galactoside | Aeromonas hydrophila | ? | - |
? | |
| additional information | does not hydrolyze 4-nitrophenyl-beta-glucoside nor 4-nitrophenyl-beta-galactoside | Aeromonas hydrophila SUWA-9 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 69000, SDS-PAGE | Aeromonas hydrophila |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NAG-B | - |
Aeromonas hydrophila |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Aeromonas hydrophila |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Aeromonas hydrophila |
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