Application | Comment | Organism |
---|---|---|
diagnostics | a spectrophotometric method for the determination of lysosomally derived N-acetyl-beta-D-hexosaminidase in synovial fluid on a microplate reader is optimized to improve its utility. The assay is sufficiently sensitive for small volumes of synovial fluid, and is useful for the clinical diagnosis of joint diseases | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
lysosome | - |
Homo sapiens | 5764 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
synovial fluid | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl N-acetyl-beta-D-glucosamine + H2O | - |
Homo sapiens | 4-nitrophenol + N-acetyl-beta-D-glucosamine | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.7 | - |
in a citrate-phosphate buffer | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 5.1 | pH 3.5: about 45% of maximal activity, pH 5.1: about 65% of maximal activity | Homo sapiens |