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Literature summary for 3.2.1.52 extracted from

  • Akeboshi, H.; Chiba, Y.; Kasahara, Y.; Takashiba, M.; Takaoka, Y.; Ohsawa, M.; Tajima, Y.; Kawashima, I.; Tsuji, D.; Itoh, K.; Sakuraba, H.; Jigami, Y.
    Production of recombinant beta-hexosaminidase A, a potential enzyme for replacement therapy for Tay-Sachs and Sandhoff diseases, in the methylotrophic yeast Ogataea minuta (2007), Appl. Environ. Microbiol., 73, 4805-4812.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
recombinant His-tagged HexA is produced in the methylotrophic yeast Ogataea minuta (alpha-1,6-mannosyltransferase-deficient). Genes encoding the alpha- and beta-subunits of HexA are integrated into the yeast cell, and the heterodimer is expressed together with its isozymes HexS (alphaalpha) and HexB (betabeta). A total of 57 mg of beta-hexosaminidase isozymes, of which 13 mg was HexA (alphabeta), is produced per liter of medium. The C terminus of the beta-subunit appears to be truncated in Ogataea minuta recombinant HexA Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Homo sapiens P07686
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme. HexA is purified with immobilized metal affinity column for the His tag attached to the beta-subunit Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.16
-
recombinant enzyme, substrate: 4-methylumbelliferyl-6-sulfo-N-acetyl-beta-D-glucosaminide Homo sapiens
0.28
-
recombinant enzyme, substrate: 4-methylumbelliferyl-N-acetyl-beta-D-glucosaminide Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-6-sulfo-N-acetyl-beta-D-glucosaminide + H2O
-
Homo sapiens ?
-
?
4-methylumbelliferyl-N-acetyl-beta-D-glucosaminide + H2O
-
Homo sapiens 4-methylumbelliferone + N-acetyl-beta-D-glucosamine
-
?

Synonyms

Synonyms Comment Organism
beta-hexosaminidase A
-
Homo sapiens