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Literature summary for 3.2.1.52 extracted from
- Purification and characterization of beta-N-acetylhexosaminidase from rice seeds (2002), J. Biochem. Mol. Biol., 35, 313-319.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetate | highest activity in phosphaute buffer and McIlvaine buffer. Activity in K-acetate buffer and K-acetate buffer is reduced to about 60% | Oryza sativa |  |
| AgNO3 | 0.1 mM, complete inhibition | Oryza sativa | |
| AlCl3 | 10 mM, 20% inhibition | Oryza sativa | |
| Chloramine T | 0.1 mM, complete inhibition | Oryza sativa | |
| CuSO4 | 10 mM, 75% inhibition | Oryza sativa | |
| glucosamine | 1 mM, 22% inhibition | Oryza sativa | |
| HgCl2 | 0.1 mM, complete inhibition | Oryza sativa | |
| iodoacetamide | 1 mM, 16% inhibition | Oryza sativa | |
| MgSO4 | 10 mM, 22% inhibition | Oryza sativa | |
| N-Acetylimidazole | 1 mM, 31% inhibition | Oryza sativa | |
| p-hydroxymercuribenzoic acid | 1 mM, 22% inhibition | Oryza sativa | |
| Pb(NO3)2 | 10 mM, 50% inhibition | Oryza sativa | |
| Phenylglyoxal | 1 mM, 20% inhibition | Oryza sativa | |
| SnCl2 | 10 mM, 44% inhibition | Oryza sativa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.17 | - |
4-nitrophenyl 2-(acetylamino)-2-deoxy-beta-D-galactopyranoside | - |
Oryza sativa | |
| 1.65 | - |
p-nitrophenyl-N-acetyl-beta-D-glucosaminide | - |
Oryza sativa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37400 | - |
gel filtration | Oryza sativa |
| 52000 | - |
x * 52000, SDS-PAGE | Oryza sativa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryza sativa | - |
L. var. Dongjin | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Oryza sativa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Oryza sativa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | no hydrolysis of chitin, chitosan, p-nitrophenyl-beta-glucopyranoside or p-nitrophenyl-beta-galactopyranoside | Oryza sativa | ? | - |
? | |
| p-nitrophenyl-N-acetyl-beta-D-galactosaminide + H2O | ratio of activity with p-nitrophenyl-N-acetyl-beta-D-glucosaminide to p-nitrophenyl-N-acetyl-beta-D-galactosaminide is 4.0. The two activities are associated with a single binding site | Oryza sativa | p-nitrophenol + N-acetyl-beta-D-galactosamine | - |
? | |
| p-nitrophenyl-N-acetyl-beta-D-glucosaminide + H2O | ratio of activity with p-nitrophenyl-N-acetyl-beta-D-glucosaminide to p-nitrophenyl-N-acetyl-beta-D-galactosaminide is 4.0. The two activities are associated with a single binding site | Oryza sativa | p-nitrophenol + N-acetyl-beta-D-glucosamine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 52000, SDS-PAGE | Oryza sativa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-HexNAcase | - |
Oryza sativa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
activity with p-nitrophenyl-N-acetyl-beta-D-glucosaminide | Oryza sativa |
| 55 | - |
activity with p-nitrophenyl-N-acetyl-beta-D-galactosaminide | Oryza sativa |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 60 | 35°C: about 40% of maximal activity, 60°C: about 50% of maximal activity, activity with p-nitrophenyl-N-acetyl-beta-D-glucosaminide | Oryza sativa |
| 40 | 60 | 40°C: about 55% of maximal activity, 60°C: about 90% of maximal activity, 70°C: no activity, activity with p-nitrophenyl-N-acetyl-beta-D-galactosaminide | Oryza sativa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 40 | 1 h, activity with p-nitrophenyl-N-acetyl-beta-D-glucosaminide and p-nitrophenyl-N-acetyl-beta-D-galactosaminide is stable at | Oryza sativa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7500 | - |
p-nitrophenyl-N-acetyl-beta-D-glalactosamine | - |
Oryza sativa | |
| 79830 | - |
p-nitrophenyl-N-acetyl-beta-D-glucosaminide | - |
Oryza sativa | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
activity with p-nitrophenyl-N-acetyl-beta-D-galactosaminide | Oryza sativa |
| 5 | - |
activity with p-nitrophenyl-N-acetyl-beta-D-glucosaminide | Oryza sativa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 5.5 | pH 3.5: about 40% of maximaol activity, pH 5.5: about 45% of maximal activity, activity with p-nitrophenyl-N-acetyl-beta-D-galactosaminide | Oryza sativa |
| 4 | 6.5 | pH 4.0: about 45% of maximal activity, pH 5.0: about 75% of maximal activity, pH 6.5: about 35% of maximaol activity, activity with p-nitrophenyl-N-acetyl-beta-D-glucosaminide | Oryza sativa |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 4.5 | 37°C, 1 h, activity with p-nitrophenyl-N-acetyl-beta-D-glalactosaminide is stable at | Oryza sativa |
| 5 | 5.5 | 37°C, 1 h, activity with p-nitrophenyl-N-acetyl-beta-D-glucosaminide is stable at | Oryza sativa |
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