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Literature summary for 3.2.1.52 extracted from
- Purification and characterization of an extracellular exochitinase, beta-N-acetylhexosaminidase, from the fungal mycoparasite Stachybotrys elegans (2002), Can. J. Microbiol., 48, 311-319.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CaCl2 | 1 mM, 13% inhibition | Stachybotrys elegans |  |
| CoCl2 | 1 mM, 15% inhibition | Stachybotrys elegans | |
| MgCl2 | 1 mM, 13% inhibition | Stachybotrys elegans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0846 | - |
p-nitrophenyl-N-acetyl-beta-D-glucosaminide | - |
Stachybotrys elegans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 68000 | - |
2 * 68000, SDS-PAGE | Stachybotrys elegans |
| 120000 | - |
gel filtration | Stachybotrys elegans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Stachybotrys elegans | the enzyme is induced when Stachybotrys elegans is grown on purified cell wall fragments of its host, Rhizoctonia solani, as well as during antagonistic interaction of the mycoparasite and host when both are grown on synthetic medium with or without supplemental carbon source | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Stachybotrys elegans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Stachybotrys elegans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 14.27 | - |
- |
Stachybotrys elegans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme is induced when Stachybotrys elegans is grown on purified cell wall fragments of its host, Rhizoctonia solani, as well as during antagonistic interaction of the mycoparasite and host when both are grown on synthetic medium with or without supplemental carbon source | Stachybotrys elegans | ? | - |
? | |
| p-nitrophenyl-N-acetyl-beta-D-glucosaminide + H2O | - |
Stachybotrys elegans | p-nitrophenol + N-acetyl-beta-D-glucosamine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 68000, SDS-PAGE | Stachybotrys elegans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| exo-acting chitinase | - |
Stachybotrys elegans |
| NAG-68 | - |
Stachybotrys elegans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Stachybotrys elegans |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 45 | 35°C: about 50% of maximal activity, 45°C: about 80% of maximal activity | Stachybotrys elegans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Stachybotrys elegans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 7 | pH 4.0: about 80% of maximal activity, pH 7.0: about 50% of maximal activity | Stachybotrys elegans |
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