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Literature summary for 3.2.1.52 extracted from

  • Reyes, F.; Byrde, R.J.
    Partial purification and properties of a beta-N-acetylglucosaminidase from the fungus Sclerotina fructigena (1973), Biochem. J., 131, 381-388.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.52

Inhibitors

Inhibitors Comment Organism Structure
N-acetyl-beta-D-glucosaminolactone
-
 Sclerotinia fructigena
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside inhibition by excess of substrate Sclerotinia fructigena

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.52
-
 N,N-diacetylchitobiose
-
 Sclerotinia fructigena
2
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
-
 Sclerotinia fructigena

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Sclerotinia fructigena
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
141000
-
 gel filtration Sclerotinia fructigena

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-beta-D-hexosaminides + H2O Sclerotinia fructigena
-
 N-acetyl-D-hexosamines
-
 ?

Organism

Organism UniProt Comment Textmining
Sclerotinia fructigena
-
-
-

Purification (Commentary)

Purification (Comment) Organism
46.7 fold to homogeneity Sclerotinia fructigena

Source Tissue

Source Tissue Comment Organism Textmining
culture filtrate
-
 Sclerotinia fructigena
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Sclerotinia fructigena

Storage Stability

Storage Stability Organism
0°C, half-life of the purified enzyme is 6 days Sclerotinia fructigena

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-galactopyranoside + H2O
-
 Sclerotinia fructigena 4-methylumbelliferone + 2-acetamido-2-deoxy-beta-D-galactopyranoside
-
 ?
4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O
-
 Sclerotinia fructigena 4-methylumbelliferone + 2-acetamido-2-deoxy-beta-D-glucopyranoside
-
 ?
chitin + H2O after 90 h at 37°C and pH 5.0, poor substrate Sclerotinia fructigena N-acetylglucosamine
-
 ?
N,N'-diacetylchitobiose + H2O
-
 Sclerotinia fructigena N-acetyl-D-glucosamine
-
 ?
N-acetyl-beta-D-hexosaminides + H2O
-
 Sclerotinia fructigena N-acetyl-D-hexosamines
-
 ?
p-nitrophenyl galactosaminide + H2O
-
 Sclerotinia fructigena p-nitrophenol + galactosamine
-
 ?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
 Sclerotinia fructigena 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
 ?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Sclerotinia fructigena 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 crude enzyme, relatively stable Sclerotinia fructigena
50
-
 50% inactivation after 10 min Sclerotinia fructigena
60
-
 complete inactivation after 10 min Sclerotinia fructigena

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.4
-
 N-acetylgalactosaminidase activity Sclerotinia fructigena
5.4
-
 N-acetylglucosaminidase activity Sclerotinia fructigena

pH Stability

pH Stability pH Stability Maximum Comment Organism
2
-
 complete inactivation after 30 min at 25°C Sclerotinia fructigena
3
-
 40% inactivation after 30 min at 25°C Sclerotinia fructigena
4 11 stable Sclerotinia fructigena