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Literature summary for 3.2.1.52 extracted from
- Purification of pig gastric-mucosal N-acetyl-beta-D-glucosaminidase (1984), Biochem. Soc. Trans., 12, 460.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| N-acetyl-D-galactosamine | PI: concentration of 30 mM causes 69% inhibition, PII: concentration of 30 mM causes 80% inhibition | Sus scrofa |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.9 | - |
4-Methylumbelliferyl beta-D-glucosaminide | PI | Sus scrofa | |
| 0.91 | - |
4-Methylumbelliferyl beta-D-glucosaminide | PII | Sus scrofa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| lysosome | - |
Sus scrofa | 5764 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acetyl-beta-D-hexosaminides + H2O | Sus scrofa | - |
N-acetyl-D-hexosamines | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sus scrofa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| forms PI and PII, each 200fold | Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| gastric mucosa | - |
Sus scrofa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl beta-D-glucosaminide + H2O | - |
Sus scrofa | 4-methylumbelliferone + D-glucosamine | - |
? | |
| 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O | - |
Sus scrofa | 4-methylumbelliferone + 2-acetamido-2-deoxy-beta-D-glucopyranoside | - |
? | |
| N-acetyl-beta-D-hexosaminides + H2O | - |
Sus scrofa | N-acetyl-D-hexosamines | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
PI: complete inactivation after 20 min, PII: after 1 h 30% of activity remain | Sus scrofa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.7 | - |
PI and PII | Sus scrofa |
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