General Stability | Organism |
---|---|
unstable to freezing and thawing | Turbatrix aceti |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | concentration of 1 mM causes 25% inhibition | Turbatrix aceti | |
acetate | concentration of 1 mM causes 30% inhibition | Turbatrix aceti | |
EDTA | concentration of 1 mM causes 40% inhibition | Turbatrix aceti | |
Fe3+ | concentration of 1 mM causes 70-80% inhibition | Turbatrix aceti | |
Hg2+ | concentration of 1 mM causes complete inhibition | Turbatrix aceti | |
p-nitrophenyl 2-deoxy-2-thioacetamido-beta-D-galactopyranoside | competitive inhibition | Turbatrix aceti | |
p-nitrophenyl 2-deoxy-2-thioacetamido-beta-D-glucopyranoside | competitive inhibition | Turbatrix aceti | |
Zn2+ | concentration of 1 mM causes 70-80% inhibition | Turbatrix aceti |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Turbatrix aceti | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
112000 | - |
native PAGE | Turbatrix aceti |
124000 | - |
gel filtration | Turbatrix aceti |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-beta-D-hexosaminides + H2O | Turbatrix aceti | - |
N-acetyl-D-hexosamines | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Turbatrix aceti | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | mannosylation | Turbatrix aceti |
glycoprotein | galactosylation | Turbatrix aceti |
glycoprotein | N-acetyl-glucosaminylation | Turbatrix aceti |
glycoprotein | fucosylation | Turbatrix aceti |
glycoprotein | N-acetyl-galactosaminylation | Turbatrix aceti |
Purification (Comment) | Organism |
---|---|
to homogeneity, 1200fold | Turbatrix aceti |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(N-acetyl-D-glucosamine)4 + 3 H2O = 4 2-(acetylamino)-2-deoxy-beta-D-glucopyranose | exo-type reaction | Turbatrix aceti |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture filtrate | - |
Turbatrix aceti | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
90 | - |
substrate: phenyl-N-acetyl-beta-D-glucosaminide | Turbatrix aceti |
Storage Stability | Organism |
---|---|
-10°C, crude culture supernatant | Turbatrix aceti |
4°C, purified enzyme, stable for several months in presence of 30% (NH4)2SO4 in 50 mM phosphate buffer, pH 7.0 | Turbatrix aceti |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
asialo-agalactofetuin + H2O | - |
Turbatrix aceti | ? | - |
? | |
chitin oligosaccharides + H2O | - |
Turbatrix aceti | N-acetyl-D-glucosamine | - |
? | |
N-acetyl-beta-D-hexosaminides + H2O | - |
Turbatrix aceti | N-acetyl-D-hexosamines | - |
? | |
ovalbumin + H2O | - |
Turbatrix aceti | N-acetylglucosamine + ? | - |
? | |
p-nitrophenyl 2-deoxy-2-thioacetamido-beta-D-galactopyranoside + H2O | poor substrate | Turbatrix aceti | 4-nitrophenol + 2-deoxy-2-thioacetamido-beta-D-galactopyranoside | - |
? | |
p-nitrophenyl 2-deoxy-2-thioacetamido-beta-D-glucopyranoside + H2O | poor substrate | Turbatrix aceti | 4-nitrophenol + 2-deoxy-2-thioacetamido-beta-D-glucopyranoside | - |
? | |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O | - |
Turbatrix aceti | 2-acetamido-2-deoxy-D-galactose + p-nitrophenol | - |
? | |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O | - |
Turbatrix aceti | 2-acetamido-2-deoxy-D-glucose + p-nitrophenol | - |
? | |
phenyl-N-acetyl-beta-D-glucosaminide + H2O | - |
Turbatrix aceti | phenol + N-acetyl-beta-D-glucosaminide | - |
? | |
Smith degradation product of human salivary glycoprotein oligosaccharide + H2O | - |
Turbatrix aceti | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | major band: 68900, minor band: 65900, SDS-PAGE | Turbatrix aceti |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.8 | - |
N-acetylglucosamnidase activity | Turbatrix aceti |
5.4 | - |
N-acetylgalactosaminidase activity | Turbatrix aceti |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.8 | - |
sharp decline of N-acetylglucosaminidase activity on either side of this value | Turbatrix aceti |
5.4 | - |
sharp decline of N-acetylgalactosaminidase activity on either side of this value | Turbatrix aceti |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | - |
stable around pH 7.0 | Turbatrix aceti |