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Literature summary for 3.2.1.52 extracted from
- Purification and properties of bovine mammary gland N-acetyl-beta-D-glucosaminidase (1985), Biochim. Biophys. Acta, 831, 125-132.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | veterinary medicine: increased level of enzyme in secreted milk can be detected by diagnosis test for mastitis of the mammary gland of cows | Bos taurus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | in milk of cows with mastitis of the mammary gland high concentrations of enzymes are found | Bos taurus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25000 | - |
A and B form: 1 * 55000 + 1 * 25000, SDS-PAGE | Bos taurus |
| 55000 | - |
A and B form: 1 * 55000 + 1 * 25000, SDS-PAGE | Bos taurus |
| 118000 | - |
A form, gel filtration | Bos taurus |
| 220000 | - |
B form, sedimentation equilibrium analysis | Bos taurus |
| 234000 | - |
B form, gel filtration | Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acetyl-beta-D-hexosaminides + H2O | Bos taurus | - |
N-acetyl-D-hexosamines | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | neutral hexose | Bos taurus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| A form, still heterogenous: 63fold, B form, close to homogeneity: 92fold | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mammary gland | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.43 | - |
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside, form A | Bos taurus |
| 2.08 | - |
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside, form B | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O | - |
Bos taurus | 4-methylumbelliferone + 2-acetamido-2-deoxy-beta-D-glucopyranoside | - |
? | |
| N-acetyl-beta-D-hexosaminides + H2O | - |
Bos taurus | N-acetyl-D-hexosamines | - |
? | |
| p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O | - |
Bos taurus | 2-acetamido-2-deoxy-D-glucose + p-nitrophenol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | A and B form: 1 * 55000 + 1 * 25000, SDS-PAGE | Bos taurus |
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Release 2023.1 (January 2023)
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