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Literature summary for 3.2.1.52 extracted from
- Characteristics of hexosaminidase A in homogenates of white blood cells using methylumbelliferyl-N-acetyl-beta-D-glucosaminide-6-sulphate as substrate (1991), Clin. Chim. Acta, 199, 231-236.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| taurocholate | concentrations of 0.05 M and 0.1 M cause 140% and 160% of activity | Homo sapiens |  |
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | diagosis of Tay-Sachs disease | Homo sapiens |
General Stability
| General Stability | Organism |
|---|---|
| resistant to mechanical inactivation by homogenization or freezing and thawing | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Hg2+ | with 0.75 M 6% of activity remains | Homo sapiens | |
| N-acetyl-D-galactosamine | - |
Homo sapiens | |
| N-acetyl-D-glucosamine | with 0.75 M 34% of activity remains | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.8 | - |
4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside-6-sulfate | - |
Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| lysosome | - |
Homo sapiens | 5764 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acetyl-beta-D-hexosaminides + H2O | Homo sapiens | - |
N-acetyl-D-hexosamines | - |
? | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| dimethyl sulfoxide | with 7.5% 14% of activity remains | Homo sapiens |
| dimethylformamide | with 7.5% 9% of activity remains | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| granulocyte | - |
Homo sapiens | - |
| leukocyte | - |
Homo sapiens | - |
| mononuclear cell | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0012 | 0.0025 | substrate: 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside-6-sulfate | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside-6-sulfate + H2O | - |
Homo sapiens | 4-methylumbelliferone + 2-acetamido-2-deoxy-beta-D-glucopyranoside-6-sulfate | - |
? | |
| N-acetyl-beta-D-hexosaminides + H2O | - |
Homo sapiens | N-acetyl-D-hexosamines | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
37 | stable up to 24 h | Homo sapiens |
| 50 | - |
rapid inactivation, retarded by acidification | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 4 | substrate: 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside-6-sulfate | Homo sapiens |
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