Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-acetamido-2-deoxy-D-galactono(1-4)lactone | with 10 mM 75% inhibition | Sus scrofa | |
D-mannose | with 10 mM 30% inhibition | Sus scrofa | |
mannosamine | with 10 mM 80% inhibition | Sus scrofa | |
methyl-alpha-D-mannoside | concentration of 10 mM causes 25% inhibition | Sus scrofa | |
additional information | - |
Sus scrofa | |
N-acetyl-beta-D-glucosaminolactone | with 10 mM 75% inhibition | Sus scrofa | |
N-acetyl-D-galactosamine | concentration of 10 mM causes 80% inhibition | Sus scrofa | |
N-acetyl-D-glucosamine | concentration of 10 mM causes 60% inhibition | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside | - |
Sus scrofa | |
1.8 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | - |
Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
160000 | - |
gel filtration | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-beta-D-hexosaminides + H2O | Sus scrofa | - |
N-acetyl-D-hexosamines | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
form A: 1300-1500fold to homogeneity | Sus scrofa |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(N-acetyl-D-glucosamine)4 + 3 H2O = 4 2-(acetylamino)-2-deoxy-beta-D-glucopyranose | same active site for N-glucosaminidase and N-galactosaminidase activities | Sus scrofa | |
(N-acetyl-D-glucosamine)4 + 3 H2O = 4 2-(acetylamino)-2-deoxy-beta-D-glucopyranose | exo-type reaction | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.1 | - |
N-acetylgalactosaminidase activity | Sus scrofa |
46.7 | - |
N-acetylglucosaminidase activity | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N,N'-diacetylchitobiose + H2O | - |
Sus scrofa | N-acetyl-D-glucosamine | - |
? | |
N-acetyl-beta-D-hexosaminides + H2O | - |
Sus scrofa | N-acetyl-D-hexosamines | - |
? | |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O | - |
Sus scrofa | 2-acetamido-2-deoxy-D-galactose + p-nitrophenol | - |
? | |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O | - |
Sus scrofa | 2-acetamido-2-deoxy-D-glucose + p-nitrophenol | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.1 | 4.3 | substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside or -glucosaminide | Sus scrofa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 7.5 | stable for 2 hr at 37°C | Sus scrofa |