Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Rhipicephalus microplus | |
Mn2+ | - |
Rhipicephalus microplus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
antibodies | inhibit about 90% of in vitro activity | Rhipicephalus microplus | |
Hg2+ | with 1 mM 13% of activity remains | Rhipicephalus microplus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Rhipicephalus microplus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
SDS-PAGE | Rhipicephalus microplus |
127000 | - |
gel filtration | Rhipicephalus microplus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-beta-D-hexosaminides + H2O | Rhipicephalus microplus | - |
N-acetyl-D-hexosamines | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhipicephalus microplus | - |
bovine tick, ixodide | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | mannosylation | Rhipicephalus microplus |
Purification (Comment) | Organism |
---|---|
to homogeneity, 1564fold | Rhipicephalus microplus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
hemolymph | - |
Rhipicephalus microplus | - |
larva | - |
Rhipicephalus microplus | - |
additional information | no activity in saliva | Rhipicephalus microplus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
77290 | - |
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | Rhipicephalus microplus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-beta-D-hexosaminides + H2O | - |
Rhipicephalus microplus | N-acetyl-D-hexosamines | - |
? | |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O | - |
Rhipicephalus microplus | 2-acetamido-2-deoxy-D-galactose + p-nitrophenol | - |
? | |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O | - |
Rhipicephalus microplus | 2-acetamido-2-deoxy-D-glucose + p-nitrophenol | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 60000, SDS-PAGE | Rhipicephalus microplus |
dimer | Hex A: alpha beta, Hex B: beta beta | Rhipicephalus microplus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Rhipicephalus microplus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
stable for 60 min at pH 4.7 | Rhipicephalus microplus |
80 | - |
completely inactivated at 80°C and above | Rhipicephalus microplus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.7 | - |
- |
Rhipicephalus microplus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | 7 | at 4°C for 24 hours and at 37°C for 60 min | Rhipicephalus microplus |