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Literature summary for 3.2.1.52 extracted from

  • Calvo, P.; Reglero, A.; Cabezas, J.A.
    Purification and properties of beta-N-acetylhexosaminidase from the mollusc Helicella ericetorum Muller (1978), Biochem. J., 175, 743-750.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
2-acetamido-2-deoxy-D-galactono(1-4)lactone complete inhibition with 100 mM Helicella ericetorum
D-mannose 75% inhibition with 100 mM Helicella ericetorum
Fe3+ concentration of 10 mM results in 85% inhibition Helicella ericetorum
Hg2+ concentration of 10 mM results in 97% inhibition Helicella ericetorum
N-acetyl-beta-D-glucosaminolactone complete inhibition with 100 mM Helicella ericetorum
N-acetyl-D-galactosamine 80% inhibition with 100 mM Helicella ericetorum
N-acetyl-D-glucosamine 80% inhibition with 100 mM Helicella ericetorum
Sodium acetate concentration of 10 mM results in 20% inhibition Helicella ericetorum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.19
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside
-
Helicella ericetorum
0.43
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
-
Helicella ericetorum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
118000 147000 gel filtration Sephadex G-200 Helicella ericetorum
120000 145000 gel filtration Sephadex G-100 Helicella ericetorum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-beta-D-hexosaminides + H2O Helicella ericetorum
-
N-acetyl-D-hexosamines
-
?

Organism

Organism UniProt Comment Textmining
Helicella ericetorum
-
Müller
-
Helicella ericetorum Muller
-
Müller
-

Purification (Commentary)

Purification (Comment) Organism
330fold Helicella ericetorum

Reaction

Reaction Comment Organism Reaction ID
(N-acetyl-D-glucosamine)4 + 3 H2O = 4 2-(acetylamino)-2-deoxy-beta-D-glucopyranose same active site for N-glucosaminidase and N-galactosaminidase activities Helicella ericetorum
(N-acetyl-D-glucosamine)4 + 3 H2O = 4 2-(acetylamino)-2-deoxy-beta-D-glucopyranose endo-type reaction Helicella ericetorum

Source Tissue

Source Tissue Comment Organism Textmining
digestive gland
-
Helicella ericetorum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.8
-
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside Helicella ericetorum
33
-
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Helicella ericetorum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitin + H2O
-
Helicella ericetorum N-acetylglucosamine
-
?
chitin + H2O
-
Helicella ericetorum Muller N-acetylglucosamine
-
?
chondroitin 4-sulfate + H2O
-
Helicella ericetorum N-acetylgalactosamine + ?
-
?
hyaluronic acid + H2O
-
Helicella ericetorum N-acetylglucosamine + ?
-
?
hyaluronic acid + H2O
-
Helicella ericetorum Muller N-acetylglucosamine + ?
-
?
N-acetyl-beta-D-hexosaminides + H2O
-
Helicella ericetorum N-acetyl-D-hexosamines
-
?
ovalbumin + H2O
-
Helicella ericetorum N-acetylglucosamine + ?
-
?
ovalbumin + H2O
-
Helicella ericetorum Muller N-acetylglucosamine + ?
-
?
ovomucoid + H2O
-
Helicella ericetorum N-acetylglucosamine + ?
-
?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
Helicella ericetorum 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
Helicella ericetorum Muller 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
Helicella ericetorum 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
Helicella ericetorum Muller 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
stable for 100 min Helicella ericetorum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside and -glucopyranoside Helicella ericetorum

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.3
-
50% of activity are lost Helicella ericetorum
3.8 4.6 at 37°C stable for 2 hours Helicella ericetorum
6.3
-
50% of activity are lost Helicella ericetorum