Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | with 0.1 mM 63% of activity remains | Halocynthia roretzi | |
Cu2+ | with 10 mM 47% of activity remains | Halocynthia roretzi | |
Hg2+ | concentration of 0.1 mM results in total loss of activity | Halocynthia roretzi | |
sodium taurodeoxycholate | - |
Halocynthia roretzi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.87 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside | - |
Halocynthia roretzi | |
1.92 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | - |
Halocynthia roretzi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
74000 | - |
SDS-PAGE | Halocynthia roretzi |
170000 | - |
at pH 7.0, gel filtration | Halocynthia roretzi |
330000 | - |
at pH 4.5, gel filtration | Halocynthia roretzi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-beta-D-hexosaminides + H2O | Halocynthia roretzi | - |
N-acetyl-D-hexosamines | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Halocynthia roretzi | - |
- |
- |
Purification (Comment) | Organism |
---|---|
820fold | Halocynthia roretzi |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
viscus | - |
Halocynthia roretzi | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
214.1 | - |
- |
Halocynthia roretzi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
asialo GM2 ganglioside + H2O | - |
Halocynthia roretzi | lactosylceramide | - |
? | |
globoside I + H2O | - |
Halocynthia roretzi | trihexaosylceramide + GalNAc | - |
? | |
GM2 ganglioside + H2O | - |
Halocynthia roretzi | GM3 ganglioside + N-acetyl-beta-D-galactosamine | - |
? | |
N-acetyl-beta-D-hexosaminides + H2O | - |
Halocynthia roretzi | N-acetyl-D-hexosamines | - |
? | |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O | - |
Halocynthia roretzi | 2-acetamido-2-deoxy-D-galactose + p-nitrophenol | - |
? | |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O | - |
Halocynthia roretzi | 2-acetamido-2-deoxy-D-glucose + p-nitrophenol | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
stable for 30 min | Halocynthia roretzi |
50 | - |
loses 30% of activity after 30 min | Halocynthia roretzi |
60 | - |
loses almost all activity after 5 min | Halocynthia roretzi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside | Halocynthia roretzi |
4.2 | - |
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | Halocynthia roretzi |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
active | Halocynthia roretzi |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 7.5 | stable | Halocynthia roretzi |
7 | - |
inactive | Halocynthia roretzi |